05-661 | Anti-β-Tubulin Antibody, clone AA2

05-661
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, BWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-661
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-β-Tubulin Antibody, clone AA2
      Alternate Names
      • Tubulin beta-5 chain
      • beta 5-tubulin
      • beta Ib tubulin
      • beta-4 tubulin
      • tubulin beta polypeptide
      • tubulin beta-1 chain
      • tubulin, beta
      • tubulin, beta polypeptide
      Background InformationMicrotubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each about 55 kDa in size.
      Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS with 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationThis Anti-β-Tubulin Antibody, clone AA2 is validated for use in WB for the detection of β-Tubulin.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPurified bovine brain tubulin. The epitope recognized by this antibody has been mapped to amino acids 412-430.
      EpitopeThe epitope has been mapped to amino acids 412-430
      CloneAA2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes β-Tubulin isotypes I, II, III, IVa, and IVb of ~50 kDa. No cross-reactivity with alpha or gamma tubulin.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      Species Reactivity NoteProven to react with human, rat, bovine, and mouse. Predicted to have broad species cross-reactivity based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • M40
      • MGC117247
      • MGC16435
      • OK/SW-cl.56
      • OTTHUMP00000029069
      • TUBB1
      • TUBB5
      • Tubb5
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07437 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 444 amino acids; 49671 Da
      SUBUNIT: Dimer of alpha and beta chains.
      TISSUE SPECIFICITY: Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
      DOMAIN: SwissProt: P07437 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from 3T3/A31, PC-12, Jurkat, or A431 cells.

      Western Blot Analysis:
      0.1-1.0 µg/mL of this lot detected β-Tubulin in RIPA lysates from 3T3/A31, PC-12, Jurkat, and A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-#946;-Tubulin, clone AA2 - 1988744 1988744
      Anti--Tubulin, clone AA2 - 2032287 2032287
      Anti--Tubulin, clone AA2 - 2207268 2207268
      Anti--Tubulin, clone AA2 - DAM1557475 DAM1557475
      Anti--Tubulin, clone AA2 - DAM1601826 DAM1601826
      Anti--Tubulin, clone AA2 - DAM1798679 DAM1798679
      Anti--Tubulin, clone AA2 - JBC1889930 JBC1889930
      Anti-b-Tubulin, clone AA2 (mouse monoclonal IgG1) - DAM1411328 DAM1411328
      Anti-b-Tubulin, clone AA2 - DAM1501797 DAM1501797
      Anti-beta;-Tubulin, clone AA2 - 22237 22237

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cumulus cells accelerate oocyte aging by releasing soluble Fas ligand in mice.
      Zhu, J; Zhang, J; Li, H; Wang, TY; Zhang, CX; Luo, MJ; Tan, JH
      Scientific reports  5  8683  2015

      Show Abstract
      25731893 25731893
      SIRT7 inactivation reverses metastatic phenotypes in epithelial and mesenchymal tumors.
      Malik, S; Villanova, L; Tanaka, S; Aonuma, M; Roy, N; Berber, E; Pollack, JR; Michishita-Kioi, E; Chua, KF
      Scientific reports  5  9841  2015

      Show Abstract
      25923013 25923013
      SIRT1 Interacts with and Deacetylates ATP6V1B2 in Mature Adipocytes.
      Kim, SY; Zhang, Q; Brunmeir, R; Han, W; Xu, F
      PloS one  10  e0133448  2015

      Show Abstract
      26177453 26177453
      Phytometabolite Dehydroleucodine Induces Cell Cycle Arrest, Apoptosis, and DNA Damage in Human Astrocytoma Cells through p73/p53 Regulation.
      Bailon-Moscoso, N; González-Arévalo, G; Velásquez-Rojas, G; Malagon, O; Vidari, G; Zentella-Dehesa, A; Ratovitski, EA; Ostrosky-Wegman, P
      PloS one  10  e0136527  2015

      Show Abstract
      Western Blotting26309132 26309132
      Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
      Yamada, Mitsutoshi, et al.
      Nature, (2014)  2014

      Show Abstract
      24776804 24776804
      SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer.
      Mazur, PK; Reynoird, N; Khatri, P; Jansen, PW; Wilkinson, AW; Liu, S; Barbash, O; Van Aller, GS; Huddleston, M; Dhanak, D; Tummino, PJ; Kruger, RG; Garcia, BA; Butte, AJ; Vermeulen, M; Sage, J; Gozani, O
      Nature  510  283-7  2014

      Show Abstract
      24847881 24847881
      Cyclin D1-Cdk4 controls glucose metabolism independently of cell cycle progression.
      Lee, Y; Dominy, JE; Choi, YJ; Jurczak, M; Tolliday, N; Camporez, JP; Chim, H; Lim, JH; Ruan, HB; Yang, X; Vazquez, F; Sicinski, P; Shulman, GI; Puigserver, P
      Nature  510  547-51  2014

      Show Abstract
      Western Blotting24870244 24870244
      Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway.
      Larios, JA; Jausoro, I; Benitez, ML; Bronfman, FC; Marzolo, MP
      BMC neuroscience  15  108  2014

      Show Abstract
      25233900 25233900
      Proteomic analysis of the SIRT6 interactome: novel links to genome maintenance and cellular stress signaling.
      Simeoni, F; Tasselli, L; Tanaka, S; Villanova, L; Hayashi, M; Kubota, K; Isono, F; Garcia, BA; Michishita-Kioi, E; Chua, KF
      Scientific reports  3  3085  2013

      Show Abstract
      Western Blotting24169447 24169447
      CCAAT enhancer binding protein δ plays an essential role in memory consolidation and reconsolidation.
      Arguello, AA; Ye, X; Bozdagi, O; Pollonini, G; Tronel, S; Bambah-Mukku, D; Huntley, GW; Platano, D; Alberini, CM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  3646-58  2013

      Show Abstract
      Immunofluorescence23426691 23426691

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      Alternative Format

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies