06-1297 | Anti-U1-70K Antibody

06-1297
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1297
      DescriptionAnti-U1-70K Antibody
      Alternate Names
      • small nuclear ribonucleoprotein 70kDa (U1)
      • U1 small nuclear ribonucleoprotein 70 kDa
      • small nuclear ribonucleoprotein 70kDa (RNP antigen)
      • U1 snRNP 70 kDa
      Background InformationU1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA.
      References
      Product Information
      FormatPurified
      Control
      • HepG2 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-U1-70K Antibody is a Rabbit Polyclonal Antibody for detection of U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1) & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 of a representative lot of this antibody has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenRecombinant protein corresponding to C-terminal domain of human U1-70K.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes U1-70K.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • U1RNP
      • U1-70K
      • U170K
      • RNPU1Z
      • SNRP70
      • U1AP1
      • RPU1
      • snRNP70
      • SNRNP70
      • U1AP
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.

      SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.

      SUBCELLULAR LOCATION: Nucleus.

      PTM: The N-terminus is blocked.

      Extensively phosphorylated on serine residues in the C-terminal region.

      MISCELLANEOUS: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.

      SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.

      SEQUENCE CAUTION: The sequence CAA28352.1 differs from that shown. Reason: Erroneous initiation.

      The sequence CAA29964.1 differs from that shown. Reason: Erroneous initiation.

      The sequence CAA29966.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight Calculated 51 kDa. Observed ~57 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HepG2 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected U1-70K on 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies