07-2130 | Anti-Ubiquitin Antibody

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedPolyclonal Antibody
      Catalogue Number07-2130
      DescriptionAnti-Ubiquitin Antibody
      Alternate Names
      • Polyubiquitin-C
      • Ubiquitin
      Background InformationUbiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein.Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.
      Product Information
      • Serum starved HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationThis Anti-Ubiquitin antibody is validated for use in IP & WB for the detection of the Ubiquitin protein.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blot Analysis: 0.5 µg/mL from a representative lot detected Ubiquitin on 10 µg of HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Ubiquitin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Ubiquitin at the N-terminus
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Broad species reactivity expected based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UBC
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~8 kDa observed. The calculated molecular weight of this protein is 8 kDa, but has been observed at ~24 kDa due to tetramers. (Kvint, K., et al. (2008). Mol Cell.30(4):498-506.)
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in serum starved HeLa cell lysate.

      Immunoprecipitation Analysis: 10 µg of this antibody detected Ubiquitin on 5 µL of serum starved HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ubiquitin 3077374
      Anti-Ubiquitin 2485467
      Anti-Ubiquitin - 2397160 2397160
      Anti-Ubiquitin - 2448421 2448421
      Anti-Ubiquitin - 2460187 2460187
      Anti-Ubiquitin - 2329169 2329169
      Anti-Ubiquitin - Q2009196 Q2009196
      Anti-Ubiquitin -2698468 2698468
      Anti-Ubiquitin -2755713 2755713
      Anti-Ubiquitin -2796243 2796243


      Reference overviewPub Med ID
      Reversal of RNA polymerase II ubiquitylation by the ubiquitin protease Ubp3.
      Kvint, Kristian, et al.
      Mol. Cell, 30: 498-506 (2008)  2008

      Show Abstract
      18498751 18498751

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      Life Science Research > Antibodies and Assays > Primary Antibodies