05-1308 | Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3, rabbit monoclonal

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbCell Function Assay, ICC, IP, IHC, WBRbPurifiedMonoclonal Antibody
      Catalogue Number05-1308
      DescriptionAnti-Ubiquitin Antibody, Lys63-Specific, clone Apu3, rabbit monoclonal
      Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388)
      Background InformationPolyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

      In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
      Product Information
      • Lys63 polyubiquitin chains.
      PresentationProtein A purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
      ApplicationAnti-Ubiquitin Antibody, Lys63-Specific, clone Apu3 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin and has been validated in Inhibition Assay (PIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC) and Western Blotting (WB).
      Key Applications
      • Cell Function Assay
      • Immunocytochemistry
      • Immunoprecipitation
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmmunoprecipitation:
      Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysates

      Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells.

      Flow cytometry:
      Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red).

      Inhibition Assay:
      Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.

      Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections.
      Biological Information
      ImmunogenImmunogen used was human di-ubiquitin linked at Lys63.
      EpitopeLys63 ubiquitination site
      SpecificityRecognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Species Reactivity NoteHuman, mouse, rabbit, rat. Based on sequence homology, broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair. Ref.21

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.

      PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.

      MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).

      SIMILARITY: Belongs to the ubiquitin family.

      Molecular Weightvaries
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ubiquitin, Lys63-Specific, clone Apu3 - 2393914 2393914
      Anti-Ubiquitin, Lys63-Specific, clone Apu3 - 2459504 2459504
      Anti-Ubiquitin, Lys63-Specific, -2517822 2517822
      Anti-Ubiquitin, Lys63-Specific, -2575910 2575910
      Anti-Ubiquitin, Lys63-Specific, -2630749 2630749
      Anti-Ubiquitin, Lys63-Specific, -2650797 2650797
      Anti-Ubiquitin, Lys63-Specific, -2664246 2664246
      Anti-Ubiquitin, Lys63-Specific, -2714551 2714551
      Anti-Ubiquitin, Lys63-Specific, -2733710 2733710
      Anti-Ubiquitin, Lys63-Specific, -2782538 2782538


      Reference overviewApplicationPub Med ID
      K63 polyubiquitination is a new modulator of the oxidative stress response.
      Silva, GM; Finley, D; Vogel, C
      Nature structural & molecular biology  22  116-23  2015

      Show Abstract
      25622294 25622294
      Coupled local translation and degradation regulate growth cone collapse.
      Deglincerti, A; Liu, Y; Colak, D; Hengst, U; Xu, G; Jaffrey, SR
      Nature communications  6  6888  2015

      Show Abstract
      25901863 25901863
      TRAF1 Coordinates Polyubiquitin Signaling to Enhance Epstein-Barr Virus LMP1-Mediated Growth and Survival Pathway Activation.
      Greenfeld, H; Takasaki, K; Walsh, MJ; Ersing, I; Bernhardt, K; Ma, Y; Fu, B; Ashbaugh, CW; Cabo, J; Mollo, SB; Zhou, H; Li, S; Gewurz, BE
      PLoS pathogens  11  e1004890  2015

      Show Abstract
      Western Blotting25996949 25996949
      Endocytic Adaptor Protein Tollip Inhibits Canonical Wnt Signaling.
      Toruń, A; Szymańska, E; Castanon, I; Wolińska-Nizioł, L; Bartosik, A; Jastrzębski, K; Miętkowska, M; González-Gaitán, M; Miaczynska, M
      PloS one  10  e0130818  2015

      Show Abstract
      26110841 26110841
      Sirt1-deficiency causes defective protein quality control.
      Tomita, T; Hamazaki, J; Hirayama, S; McBurney, MW; Yashiroda, H; Murata, S
      Scientific reports  5  12613  2015

      Show Abstract
      26219988 26219988
      Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
      Guicciardi, ME; Werneburg, NW; Bronk, SF; Franke, A; Yagita, H; Thomas, G; Gores, GJ
      PloS one  9  e92124  2014

      Show Abstract
      24633224 24633224
      Cell fate decisions regulated by K63 ubiquitination of tumor necrosis factor receptor 1.
      Fritsch, J; Stephan, M; Tchikov, V; Winoto-Morbach, S; Gubkina, S; Kabelitz, D; Schütze, S
      Molecular and cellular biology  34  3214-28  2014

      Show Abstract
      24980434 24980434
      Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis antagonizing function of XIAP E3 ligase.
      Hamacher-Brady, A; Choe, SC; Krijnse-Locker, J; Brady, NR
      Cell death and differentiation  21  1862-76  2014

      Show Abstract
      25080938 25080938
      Differential roles of the ubiquitin proteasome system and autophagy in the clearance of soluble and aggregated TDP-43 species.
      Scotter, EL; Vance, C; Nishimura, AL; Lee, YB; Chen, HJ; Urwin, H; Sardone, V; Mitchell, JC; Rogelj, B; Rubinsztein, DC; Shaw, CE
      Journal of cell science  127  1263-78  2014

      Show Abstract
      24424030 24424030
      Dimerization and ubiquitin mediated recruitment of A20, a complex deubiquitinating enzyme.
      Lu, TT; Onizawa, M; Hammer, GE; Turer, EE; Yin, Q; Damko, E; Agelidis, A; Shifrin, N; Advincula, R; Barrera, J; Malynn, BA; Wu, H; Ma, A
      Immunity  38  896-905  2013

      Show Abstract
      Western Blotting23602765 23602765


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      Life Science Research > Antibodies and Assays > Primary Antibodies