05-1313 Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4

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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtELISA, IHC, ICC, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-1313
      DescriptionAnti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4
      Alternate Names
      • ubiquitin C
      • polyubiquitin B
      • ubiquitin B
      Background InformationPolyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
      Product Information
      • Lys63 polyubiquitin chains.
      PresentationPurified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
      ApplicationAnti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
      Additional Research Applications

      This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.

      This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer's patient.

      This antibody has been reported by an outside laboratory to be suitable for ELISA.

      Biological Information
      ImmunogenImmunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).
      EpitopeLys63 ubiquitination site
      SpecificityRecognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteHuman. Based on sequence homology, broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. [provided by RefSeq]
      Gene Symbol
      • UBB
      • UBC
      • FLJ25987
      • MGC8385
      • OTTHUMP00000064960
      • OTTHUMP00000064961
      • UBA80
      • UBCEP1
      • UBCEP2
      UniProt Number
      UniProt SummaryFUNCTION: Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
      PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
      MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
      SIMILARITY: Belongs to the ubiquitin family.
      Molecular Weightvaries
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 - 24247272424727
      Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 - 24501872450187
      Anti-Ubiquitin, Lys63-Specific, -26045532604553
      Anti-Ubiquitin, Lys63-Specific, -26944592694459
      Anti-Ubiquitin, Lys63-Specific, -27936272793627
      Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 - 19837101983710
      Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 - 20094542009454
      Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 - 20609622060962
      Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 - 22103532210353
      Anti-Ubiquitin, Lys63-Specific, clone HWA4C4 - 23274612327461


      Reference overviewPub Med ID
      Polyubiquitination of Transforming Growth Factor β-activated Kinase 1 (TAK1) at Lysine 562 Residue Regulates TLR4-mediated JNK and p38 MAPK Activation.
      Chen, IT; Hsu, PH; Hsu, WC; Chen, NJ; Tseng, PH
      Scientific reports  5  12300  2015

      Show Abstract
      26189595 26189595
      SKP2 inactivation suppresses prostate tumorigenesis by mediating JARID1B ubiquitination.
      Lu, W; Liu, S; Li, B; Xie, Y; Adhiambo, C; Yang, Q; Ballard, BR; Nakayama, KI; Matusik, RJ; Chen, Z
      Oncotarget  6  771-88  2015

      Show Abstract
      25596733 25596733
      TRIM30α Is a Negative-Feedback Regulator of the Intracellular DNA and DNA Virus-Triggered Response by Targeting STING.
      Wang, Y; Lian, Q; Yang, B; Yan, S; Zhou, H; He, L; Lin, G; Lian, Z; Jiang, Z; Sun, B
      PLoS pathogens  11  e1005012  2015

      Show Abstract
      26114947 26114947
      HTLV-1 tax stabilizes MCL-1 via TRAF6-dependent K63-linked polyubiquitination to promote cell survival and transformation.
      Choi, YB; Harhaj, EW
      PLoS pathogens  10  e1004458  2014

      Show Abstract
      25340740 25340740
      An unexpected twist to the activation of IKKβ: TAK1 primes IKKβ for activation by autophosphorylation.
      Zhang, J; Clark, K; Lawrence, T; Peggie, MW; Cohen, P
      The Biochemical journal  461  531-7  2014

      Show Abstract
      24911653 24911653
      The E3 ubiquitin ligase TRIM21 negatively regulates the innate immune response to intracellular double-stranded DNA.
      Zhang, Z; Bao, M; Lu, N; Weng, L; Yuan, B; Liu, YJ
      Nature immunology  14  172-8  2013

      Show Abstract
      23222971 23222971
      Cellular inhibitors of apoptosis proteins cIAP1 and cIAP2 are required for efficient caspase-1 activation by the inflammasome.
      Labbé, K; McIntire, CR; Doiron, K; Leblanc, PM; Saleh, M
      Immunity  35  897-907  2011

      Show Abstract
      22195745 22195745
      Lys63-linked polyubiquitin chains: linking more than just ubiquitin.
      Kawadler, Holli and Yang, Xiaolu
      Cancer Biol. Ther., 5: 1273-4 (2006)  2006

      Show Abstract
      16969079 16969079
      Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein.
      Galan, J M and Haguenauer-Tsapis, R
      EMBO J., 16: 5847-54 (1997)  1997

      Show Abstract
      9312043 9312043


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      Life Science Research > Antibodies and Assays > Primary Antibodies