04-454 | Anti-Ubiquitin (CT) Antibody, rabbit monoclonal

04-454
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-454
      Replaces07-483; AB5621
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ubiquitin (CT) Antibody, rabbit monoclonal
      References
      Product Information
      FormatPurified
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Ubiquitin (C-term) using this Anti-Ubiquitin (C-term) Antibody, Rabbit validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide composed of residues at the C-terminus of human Ubiquitin.
      HostRabbit
      SpecificityRecognizes a region composed of residues at the C-terminus of human Ubiquitin.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
      Gene Symbol
      • RPS27A
      • CEP80
      • ubiquitin
      • ubiquitin-CEP80
      • UBCEP1
      • Ubiquitin.
      • UBA52
      • UBCEP2
      • UBA80
      • UBC
      • UBB
      • HUBCEP80
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
      SIZE: 76 amino acids; 8565 Da
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
      PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
      SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
      MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
      Molecular Weight8.5kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ubiquitin (C-term) 2470372
      Anti-Ubiquitin (C-term) (Rabbit monoclonal IgG) - 1993750 1993750
      Anti-Ubiquitin (C-term) - 2020273 2020273
      Anti-Ubiquitin (C-term) - 2051318 2051318
      Anti-Ubiquitin (C-term) - DAM1425002 DAM1425002
      Anti-Ubiquitin (C-term) - DAM1691570 DAM1691570
      Anti-Ubiquitin (C-term) - NG1685124 NG1685124
      Anti-Ubiquitin (C-term) - NG1845652 NG1845652
      Anti-Ubiquitin (C-term) -2526726 2526726
      Anti-Ubiquitin (C-term), Rabbit Monoclonal - 0611046358 0611046358

      References

      Reference overviewPub Med ID
      Individual Src-family tyrosine kinases direct the degradation or protection of the clock protein Timeless via differential ubiquitylation.
      O'Reilly, LP; Zhang, X; Smithgall, TE
      Cellular signalling  25  860-6  2013

      Show Abstract
      23266470 23266470
      Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini.
      Ouyang, H; Ali, YO; Ravichandran, M; Dong, A; Qiu, W; MacKenzie, F; Dhe-Paganon, S; Arrowsmith, CH; Zhai, RG
      The Journal of biological chemistry  287  2317-27  2012

      Show Abstract
      22069321 22069321
      Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1.
      Shinichiro Nakada,Ikue Tai,Stephanie Panier,Abdallah Al-Hakim,Shun-Ichiro Iemura,Yu-Chi Juang,Lara O'Donnell,Ayako Kumakubo,Meagan Munro,Frank Sicheri,Anne-Claude Gingras,Tohru Natsume,Toshio Suda,Daniel Durocher
      Nature  466  2010

      Show Abstract
      20725033 20725033
      Multiple forms of ubiquitin-activating enzyme E1 from wheat. Identification of an essential cysteine by in vitro mutagenesis
      Hatfield, P M and Vierstra, R D
      J Biol Chem, 267:14799-803 (1992)  1992

      1634524 1634524
      Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells
      Goldstein, G, et al
      Proc Natl Acad Sci USA, 72:11-5 (1975)  1975

      1078892 1078892

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies