05-1307-AF488 | Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate

05-1307-AF488
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICCRbAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-1307-AF488
      Description Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate
      Alternate Names
      • Polyubiquitin-B
      • Ubiquitin, Lys48-Specific
      • Ubiquitin
      Background InformationPolyubiquitin-B (UniProt P0CG47) is encoded by the UBB gene (Gene ID 7314) in human. Ubiqutin (Ub) is initially produced as a 229-a.a. precursor protein whose C-terminus Cys229 is post-translationally removed, followed by cleavages between a.a. 76 and 77, as well as between a.a. 152 and 153 to yield three idential 76-amino acid Ub molecules. Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyubiquitin chain, ubiquitin molecules can be linked through one of the seven ubiquitin Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Ubiquitin, Lys48-Specific, clone Apu2, Alexa Fluor® 488 conjugate is validated for use in Immunocytochemistry for the detection of Ubiquitin.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Ubiquitin in A431 and NIH/3T3 cells.
      The unconjugated antibody (Cat. No. 05-1307) is shown to be suitable also for cell funtion assay, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, Western blotting applications.
      Biological Information
      ImmunogenImmunogen used was human di-ubiquitin linked at Lys48.
      EpitopeLys48 ubiquitination site
      CloneApu2
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes ubiquitin linked at Lys48. Does not recognize ubiquitin linked at Lys63 or free ubiquitin.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Rabbit based on 100% sequence homology. Based on sequence homology, broad species cross-reactivity is expected. .
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • UBB
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ubiquitin in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies