09-722 | Anti-XBP1 Antibody

09-722
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-722
      DescriptionAnti-XBP1 Antibody
      Alternate Names
      • Tax-responsive element-binding protein 5
      • X-box binding protein 1
      • X-box-binding protein-1
      Background InformationX-box Protein 1 (XBP1) is a transcription factor that belongs to the bZIP family. XBP1 plays an important role in the functioning of the immune system and in the cellular stress response. It is essential for the differentiation of plasma cells and is involved in the regulation of the MHC class II genes. XBP1 becomes initiated during an endoplasmic reticulum (ER) stress response by binding to the unfolded protein response element (UPRE) which activates the UPR target genes. Mutations in XBP1 have been associated with mental disorders.
      References
      Product Information
      FormatAffinity Purified
      Control
      • LNCAP cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-XBP1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect XBP1 also known as X-box binding protein 1.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: 6 µg/ml from a representative lot detected XBP1 on 10 µg of LNCAP cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human XBP1 at and around the N-terminus.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes XBP1 at the C-terminus.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Peptide homology with rat and mouse is 86%.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
      Gene Symbol
      • XBP1
      • TREB5
      • XBP-1
      • XBP2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N3[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.

      SUBCELLULAR LOCATION: Nucleus.

      INDUCTION: Up-regulated by ATF6 via direct binding to the ERSE in response to endoplasmic reticulum stress.

      INVOLVEMENT IN DISEASE: Genetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.

      SEQUENCE SIMILARITIES: Belongs to the bZIP family.

      Contains 1 bZIP domain
      Molecular Weight 29 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in LNCAP cell lysate.

      Western Blot Analysis: 2 µg/ml of this antibody detected XBP1 on 10 µg of LNCAP cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies