07-1614 | Anti-XRCC2 Antibody

07-1614
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIH(P), WB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1614
      ReplacesMABE556
      DescriptionAnti-XRCC2 Antibody
      Alternate Names
      • DNA repair protein XRCC2
      • X-ray repair complementing defective repair in Chinese hamster cells 2
      • X-ray repair cross complementing protein 2
      • X-ray repair cross-complementing protein 2
      • X-ray repair
      • complementing defective
      • repair in Chinese hamster
      Background InformationX-ray repair cross-complementing protein (XRCC2) is a member of the RecA family which is essential for DNA repair and maintenance. XRCC2 also belongs to the Rad51 subfamily of proteins involved in double stranded DNA break repair. XRCC2 is thought to encode a member of the RecA/Rad51 related protein family which maintains chromosome stability and repairs DNA damage. XRCC2 and RAD51 interact to form a multimeric ring in the absence of DNA. XRCC2 is thought to load Rad51 onto gapped DNA.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate, human lung, NIH/3T3, A431, HeLa cells.
      PresentationRabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives
      Applications
      ApplicationUse Anti-XRCC2 Antibody (rabbit polyclonal antibody) validated in IHC(P), WB, ICC to detect XRCC2 also known as DNA repair protein XRCC2, X-ray repair complementing defective repair in Chinese hamster cells 2.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry:
      Anti-XRCC2 polyclonal was used in immunocytochemistry to positively stain the nucleus on A431, NIH/3T3 and HeLa cells.

      Immunohistochemistry(paraffin): Anti-XRCC2 antibody was used for immunohistochemistry on human lung tissue.
      Formalin-fixed and paraffin-embedded Human lung tissue stained with anti-XRCC2 (1:100) with Citrate pH 6.0 (HIER).
      Biological Information
      ImmunogenA synthetic peptide corresponding to amino acids 206-217 of XRCC2.
      HostRabbit
      SpecificityThis antibody recognizes XRCC2.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents. [provided by RefSeq].
      Gene Symbol
      • DKFZp781P0919
      • RAD51-like
      Purification MethodAffinity purified polyclonal precipitated in ammonium sulfate.
      UniProt Number
      UniProt SummaryFUNCTION: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Ref.6

      SUBUNIT STRUCTURE: Interacts with RAD51D. Part of a BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2. Part of a complex consisting of RAD51B, RAD51C, RAD51D, XRCC2 and XRCC3. In the absence of DNA, XRCC2-RAD51D formed a multimeric ring structure. In the presence of single-stranded DNA, XRCC2-RAD51D formed a filamentous structure.

      SUBCELLULAR LOCATION: Nucleus.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.10

      SIMILARITY: Belongs to the recA family. RAD51 subfamily.

      Molecular Weight~32 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      1:500 dilution of this antibody detected XRCC2 in 10 µg of a Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications