05-1483 | Anti-Zizimin1 Antibody, clone 8B3-C3-E4

05-1483
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1483
      DescriptionAnti-Zizimin1 Antibody, clone 8B3-C3-E4
      Alternate Names
      • Cdc42 guanine nucleotide exchange factor zizimin-1
      • dedicator of cytokinesis 9
      Background InformationZizimin1, also known as DOCK 9 (dedicator of cytokinesis 9), is involved in intracellular signalling networks by functioning as a guanine nucleotide-exchange factor (GEF). Zizimin1 activates Cdc42, a Rho family GTPase that is involved in cell polarity, gene expression, cell-cycle progression and cell-cell interactions.
      References
      Product Information
      FormatPurified
      Control
      • HUVEC cell lysate.
      PresentationPurified mouse monoclonal supernatant in 0.1M Tris-Glycine (pH 7.4) with 150 mM NaCl with 0.05% NaN3.
      Applications
      ApplicationThis Anti-Zizimin1 Antibody, clone 8B3-C3-E4 is validated for use in WB, IC, IP for the detection of Zizimin1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry:
      Biological Information
      ImmunogenFull length human recombinant Zizimin1.
      EpitopeFull Length
      Clone8B3-C3-E4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes human Zizimin1, clone 8B3-C3-E4.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DKFZp686C11110
      • DKFZp686D2047
      • DKFZp686N04132
      • FLJ11949
      • FLJ16744
      • FLJ44528
      • FLJ45282
      • FLJ45601
      • KIAA1058
      • KIAA1085
      • OTTHUMP00000040724
      • ZIZ1
      • ZIZIMIN1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.

      SUBUNIT STRUCTURE: Interacts preferentially with nucleotide-depleted CDC42.

      SUBCELLULAR LOCATION: Endomembrane system Probable. Note: Associated with membranes Probable.

      TISSUE SPECIFICITY: Widely expressed, with highest expression in heart and placenta. Expressed at intermediate level in kidney, brain, lung and skeletal muscle.

      DOMAIN: The DHR-2 domain is necessary and sufficient for the GEF activity.

      MISCELLANEOUS: 'Zizim' means 'spike' in Hebrew.

      SEQUENCE SIMILARITIES: Belongs to the DOCK family.

      Contains 1 DHR-1 (CZH-1) domain.

      Contains 1 DHR-2 (CZH-2) domain.

      Contains 1 PH domain.

      Molecular Weight236 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on HUVEC cell lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected Zizimin1 in lysate from HUVEC cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications