05-1098 | Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2

05-1098
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1098
      Replaces04-322
      DescriptionAnti-acetyl CoA Carboxylase Antibody, clone 7D2.2
      Alternate Names
      • acetyl-CoA carboxylase 1
      • acetyl-CoA carboxylase-alpha
      • acetyl-Coenzyme A carboxylase alpha
      Background InformationAcetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activity of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormone glucagon or epinephrine binds to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.
      References
      Product Information
      FormatPurified
      Control
      • Human heart lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-acetyl CoA Carboxylase Antibody, clone 7D2.2 detects level of acetyl CoA Carboxylase & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of rat heart lysate.

      Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human acetyl CoA Carboxylase.
      Clone7D2.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAcetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriche in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
      Gene Symbol
      • ACAC
      • ACC
      • ACC-alpha
      • ACC1
      • ACCA
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

      CATALYTIC ACTIVITY: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.

      ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.

      COFACTOR: Biotin.

      Binds 2 manganese ions per subunit.

      ENZYME REGULATION: By phosphorylation (By similarity).

      PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.

      SUBUNIT STRUCTURE: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.

      SUBCELLULAR LOCATION: Cytoplasm.

      TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

      PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.

      INVOLVEMENT IN DISEASE: Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth.

      SEQUENCE SIMILARITIES: Contains 1 ATP-grasp domain.

      Contains 1 biotin carboxylation domain.

      Contains 1 biotinyl-binding domain.

      Contains 1 carboxyltransferase domain.
      Molecular Weight~ 266 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human heart lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl CoA Carboxylase, clone 7D2.2 - 2140083 2140083
      Anti-acetyl CoA Carboxylase, clone 7D2.2 - 2398930 2398930
      Anti-acetyl CoA Carboxylase, clone 7D2.2 - NRG1764424 NRG1764424
      Anti-acetyl CoA Carboxylase, -2514242 2514242
      Anti-acetyl CoA Carboxylase, -2550885 2550885
      Anti-acetyl CoA Carboxylase, -2631522 2631522
      Anti-acetyl CoA Carboxylase, -2730929 2730929
      Anti-acetyl CoA Carboxylase, -2814761 2814761
      Anti-acetyl CoA Carboxylase, clone 7D2.2 2890782
      Anti-acetyl CoA Carboxylase, clone 7D2.2 - 1990710 1990710

      References

      Reference overviewPub Med ID
      Human acetyl-CoA carboxylase 1 gene: presence of three promoters and heterogeneity at the 5'-untranslated mRNA region.
      Mao, Jianqiang, et al.
      Proc. Natl. Acad. Sci. U.S.A., 100: 7515-20 (2003)  2003

      Show Abstract
      12810950 12810950

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies