06-599-AF647 | Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate

06-599-AF647
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCRbAlexaFluor®647Polyclonal Antibody
      Description
      Catalogue Number06-599-AF647
      DescriptionAnti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate
      Alternate Names
      • H3 histone family, member T
      • Histone 3, H3
      • Histone cluster 3, H3
      Background InformationHistone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. The chromatin fiber is composed on repetitive units, known as nucleosomes, which are comprised of an octamer of core histones (two each of H2A, H2B, H3, and H4), around which DNA are wrapped. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified rabbit antibody conjugate in buffer containing PBS, 15 mg/mL BSA and 0.1% Sodium Azide.
      Applications
      ApplicationThis Anti-acetyl-Histone H3 Antibody, Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of acetyl-Histone H3.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H3 in NIH/3T3 cells.
      Biological Information
      ImmunogenSynthetic peptide corresponding to human histone H3 N-terminal sequence with acetylated Lys9 and Lys14
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityAntibody recognizes histone H3 with acetylated Lys9 and/or Lys14.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology. Other homologies: Broad species cross-reactivity expected based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Modifications
      • Acetylation
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H3 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3, Alexa Fluor® -2800494 2800494
      Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate 2884279
      Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate -2736368 2736368
      Anti-acetyl-Histone H3, Alexa Fluor® 647 Conjugate -Q2586390 Q2586390

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.