06-911 | Anti-acetyl-Histone H3 (Lys14) Antibody

06-911
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, EuICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-911
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys14) Antibody
      Alternate Names
      • H3K14Ac
      • Histone H3 (acetyl K14)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      Control
      • Sodium butyrate treated acid extracted proteins from HeLa cells.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide and 30% glycerol. Store at -20°C.
      Applications
      ApplicationUse Anti-acetyl-Histone H3 (Lys14) Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14).
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      1 μg of a previous lot immunoprecipitated acetylated Histone-H3 from 500 μg of an acid precipitate of sodium butyrate treated HeLa cells.

      Immunocytochemistry:
      Not determined.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide (ARKSTGG[AcK]APR-GC) corresponding to amino acids 7-17 of Histone-H3.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone-H3, Mr 17 kDa, acetylated at position 14.
      IsotypeIgG
      Species Reactivity
      • Human
      • Eukaryote
      Species Reactivity NoteHuman. This peptide sequence is identical in a wide range of animal and plant species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3/t
      • H3t
      • MGC126888
      • H3/g
      • H3.4
      • H3T
      • MGC126886
      Modifications
      • Acetylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular WeightApprox. 17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on sodium butyrate treated acid extracted proteins from HeLa cells.

      Western Blot Analysis:
      0.5-1.0 μg/mL of this lot detected acetylated Histone-H3 in acid extracted proteins from HeLa cells. Sodium butyrate, an inhibitor of histone deacetylases, was used to enhance detection of acetylated Histone-H3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys14) - 2424729 2424729
      Anti-acetyl-Histone H3 (Lys14) - 2462852 2462852
      Anti-acetyl-Histone H3 (Lys14) - 18439 18439
      Anti-acetyl-Histone H3 (Lys14) - 1971846 1971846
      Anti-acetyl-Histone H3 (Lys14) - 2019744 2019744
      Anti-acetyl-Histone H3 (Lys14) - 2073116 2073116
      Anti-acetyl-Histone H3 (Lys14) - 21626 21626
      Anti-acetyl-Histone H3 (Lys14) - 2189949 2189949
      Anti-acetyl-Histone H3 (Lys14) - 22854 22854
      Anti-acetyl-Histone H3 (Lys14) - 2377183 2377183

      References

      Reference overviewApplicationSpeciesPub Med ID
      The Drosophila Su(var)3-7 gene is required for oogenesis and female fertility, genetically interacts with piwi and aubergine, but impacts only weakly transposon silencing.
      Basquin, D; Spierer, A; Begeot, F; Koryakov, DE; Todeschini, AL; Ronsseray, S; Vieira, C; Spierer, P; Delattre, M
      PloS one  9  e96802  2014

      Show Abstract
      Immunofluorescence24820312 24820312
      Neural stem cell differentiation is dictated by distinct actions of nuclear receptor corepressors and histone deacetylases.
      Castelo-Branco, G; Lilja, T; Wallenborg, K; Falcão, AM; Marques, SC; Gracias, A; Solum, D; Paap, R; Walfridsson, J; Teixeira, AI; Rosenfeld, MG; Jepsen, K; Hermanson, O
      Stem cell reports  3  502-15  2014

      Show Abstract
      25241747 25241747
      Lysine acetyltransferase GCN5b interacts with AP2 factors and is required for Toxoplasma gondii proliferation.
      Wang, J; Dixon, SE; Ting, LM; Liu, TK; Jeffers, V; Croken, MM; Calloway, M; Cannella, D; Hakimi, MA; Kim, K; Sullivan, WJ
      PLoS pathogens  10  e1003830  2014

      Show Abstract
      24391497 24391497
      Molecular pathways underpinning ethanol-induced neurodegeneration.
      Goldowitz, D; Lussier, AA; Boyle, JK; Wong, K; Lattimer, SL; Dubose, C; Lu, L; Kobor, MS; Hamre, KM
      Frontiers in genetics  5  203  2014

      Show Abstract
      Western BlottingMouse25076964 25076964
      Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity.
      Lau, PN; Cheung, P
      Nucleic acids research  41  e49  2013

      Show Abstract
      23258705 23258705
      Histone deacetylase inhibitors facilitate partner preference formation in female prairie voles.
      Wang, H; Duclot, F; Liu, Y; Wang, Z; Kabbaj, M
      Nature neuroscience  16  919-24  2013

      Show Abstract
      Western Blotting23727821 23727821
      The histone deacetylase inhibitor sodium butyrate modulates acquisition and extinction of cocaine-induced conditioned place preference.
      Raybuck, JD; McCleery, EJ; Cunningham, CL; Wood, MA; Lattal, KM
      Pharmacology, biochemistry, and behavior  106  109-16  2013

      Show Abstract
      Immunofluorescence23454534 23454534
      MYCN and HDAC2 cooperate to repress miR-183 signaling in neuroblastoma.
      Lodrini, M; Oehme, I; Schroeder, C; Milde, T; Schier, MC; Kopp-Schneider, A; Schulte, JH; Fischer, M; De Preter, K; Pattyn, F; Castoldi, M; Muckenthaler, MU; Kulozik, AE; Westermann, F; Witt, O; Deubzer, HE
      Nucleic acids research  41  6018-33  2013

      Show Abstract
      23625969 23625969
      Functional characterization and gene expression profiling of Drosophila melanogaster short dADA2b isoform-containing dSAGA complexes.
      Pankotai, T; Zsindely, N; Vamos, EE; Komonyi, O; Bodai, L; Boros, IM
      BMC genomics  14  44  2013

      Show Abstract
      23336284 23336284
      Role of DNA methylation in the regulation of lipogenic glycerol-3-phosphate acyltransferase 1 gene expression in the mouse neonatal liver.
      Ehara, T; Kamei, Y; Takahashi, M; Yuan, X; Kanai, S; Tamura, E; Tanaka, M; Yamazaki, T; Miura, S; Ezaki, O; Suganami, T; Okano, M; Ogawa, Y
      Diabetes  61  2442-50  2012

      Show Abstract
      22721968 22721968

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