06-942 | Anti-acetyl-Histone H3 (Lys9) Antibody

06-942
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ChIP, DB, FC, ChIP-seqRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-942
      Replaces04-1003
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H3 (Lys9) Antibody
      Alternate Names
      • H3K9Ac
      • Histone H3 (acetyl K9)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
      Applications
      ApplicationAnti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot blot (DB), published in peer reviewed journals, and validated in WB, ChIP, ChIP-seq, FC.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Dot Blot
      • Flow Cytometry
      • ChIP-seq
      Application NotesChromatin Immunoprecipitation/ChIP-seq Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys9) in 5 µg of HeLa cell lysate.
      Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.
      EpitopeSurrounding H3K9ac
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityHistone H3 acetylated at position 9.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat, although this peptide sequence is identical in a wide range of animal and plant species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      • HIST3H3
      • H3FT
      • H3T
      • MGC126886
      Modifications
      • Acetylation
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.

      Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys9) - 2135172 2135172
      Anti-acetyl-Histone H3 (Lys9) - 18740 18740
      Anti-acetyl-Histone H3 (Lys9) - 1973414 1973414
      Anti-acetyl-Histone H3 (Lys9) - 2111283 2111283
      Anti-acetyl-Histone H3 (Lys9) - 2178419 2178419
      Anti-acetyl-Histone H3 (Lys9) - 21897 21897
      Anti-acetyl-Histone H3 (Lys9) - 2193154 2193154
      Anti-acetyl-Histone H3 (Lys9) - 2279510 2279510
      Anti-acetyl-Histone H3 (Lys9) - 23997 23997
      Anti-acetyl-Histone H3 (Lys9) - 2511087 2511087

      References

      Reference overviewApplicationSpeciesPub Med ID
      Transcriptional regulation of the human TNFSF11 gene in T cells via a cell type-selective set of distal enhancers.
      Bishop, KA; Wang, X; Coy, HM; Meyer, MB; Gumperz, JE; Pike, JW
      Journal of cellular biochemistry  116  320-30  2015

      Show Abstract
      25211367 25211367
      MAF1 represses CDKN1A through a Pol III-dependent mechanism.
      Lee, YL; Li, YC; Su, CH; Chiao, CH; Lin, IH; Hsu, MT
      eLife  4  e06283  2015

      Show Abstract
      26067234 26067234
      A comprehensive epigenome map of Plasmodium falciparum reveals unique mechanisms of transcriptional regulation and identifies H3K36me2 as a global mark of gene suppression.
      Karmodiya, K; Pradhan, SJ; Joshi, B; Jangid, R; Reddy, PC; Galande, S
      Epigenetics & chromatin  8  32  2015

      Show Abstract
      26388940 26388940
      Hierarchical clustering of breast cancer methylomes revealed differentially methylated and expressed breast cancer genes.
      Lin, IH; Chen, DT; Chang, YF; Lee, YL; Su, CH; Cheng, C; Tsai, YC; Ng, SC; Chen, HT; Lee, MC; Chen, HW; Suen, SH; Chen, YC; Liu, TT; Chang, CH; Hsu, MT
      PloS one  10  e0118453  2015

      Show Abstract
      25706888 25706888
      Deep sequencing and de novo assembly of the mouse oocyte transcriptome define the contribution of transcription to the DNA methylation landscape.
      Veselovska, L; Smallwood, SA; Saadeh, H; Stewart, KR; Krueger, F; Maupetit-Méhouas, S; Arnaud, P; Tomizawa, S; Andrews, S; Kelsey, G
      Genome biology  16  209  2015

      Show Abstract
      26408185 26408185
      A single allele of Hdac2 but not Hdac1 is sufficient for normal mouse brain development in the absence of its paralog.
      Hagelkruys, A; Lagger, S; Krahmer, J; Leopoldi, A; Artaker, M; Pusch, O; Zezula, J; Weissmann, S; Xie, Y; Schöfer, C; Schlederer, M; Brosch, G; Matthias, P; Selfridge, J; Lassmann, H; Knoblich, JA; Seiser, C
      Development (Cambridge, England)  141  604-16  2014

      Show Abstract
      Immunohistochemistry24449838 24449838
      Dynamic remodeling of histone modifications in response to osmotic stress in Saccharomyces cerevisiae.
      Magraner-Pardo, L; Pelechano, V; Coloma, MD; Tordera, V
      BMC genomics  15  247  2014

      Show Abstract
      24678875 24678875
      Lysine acetyltransferase GCN5b interacts with AP2 factors and is required for Toxoplasma gondii proliferation.
      Wang, J; Dixon, SE; Ting, LM; Liu, TK; Jeffers, V; Croken, MM; Calloway, M; Cannella, D; Hakimi, MA; Kim, K; Sullivan, WJ
      PLoS pathogens  10  e1003830  2014

      Show Abstract
      24391497 24391497
      Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines.
      Maroschik, B; Gürtler, A; Krämer, A; Rößler, U; Gomolka, M; Hornhardt, S; Mörtl, S; Friedl, AA
      Radiation oncology (London, England)  9  15  2014

      Show Abstract
      Western Blotting24406105 24406105
      CDK2-dependent phosphorylation of Suv39H1 is involved in control of heterochromatin replication during cell cycle progression.
      Park, SH; Yu, SE; Chai, YG; Jang, YK
      Nucleic acids research  42  6196-207  2014

      Show Abstract
      24728993 24728993

      Technical Info

      Title
      Anti-acetyl-Histone H3 (Lys9) ChIP-Seq Analysis
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies