04-1003 | Anti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal

04-1003
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IH(P), IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1003
      DescriptionAnti-acetyl-Histone H3 (Lys9) Antibody, clone Y28, rabbit monoclonal
      Alternate Names
      • H3K9Ac
      • Histone H3 (acetyl K9)
      • H3 histone, family 3A
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatUnpurified
      Control
      • Untreated and TSA-treated NIH/3T3 cell lysate.
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Histone H3 in human lung carcinoma tissue.
      Immunocytochemistry Analysis: A 1:250 dilution from a previous lot was used in IC.
      Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenAcetylated-synthetic peptide corresponding to residues surrounding Lys9 of Histone H3.
      EpitopeAcetylated Lys9
      CloneY28
      HostRabbit
      SpecificityThe antibody only detects Histone H3 acetylated on Lys9.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H3.3A
      • H3.3B
      • H3F3
      • MGC87782
      • MGC87783
      • OTTHUMP00000035618
      • OTTHUMP00000035619
      • OTTHUMP00000035621
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes.
      SUBCELLULAR LOCATION: Nucleus.
      DEVELOPMENTAL STAGE: Expressed throughout the cell cycle independently of DNA synthesis.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
      Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
      Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
      Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5 (H3K4me), Lys-37 and Lys-80. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me), which are linked to gene repression, are underrepresented. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
      Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, probably DAPK3. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes.
      Ubiquitinated By similarity.
      SEQUENCE SIMILARITIES: Belongs to the histone H3 family.
      SEQUENCE CAUTION: The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight~17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and TSA-treated NIH/3T3 cell lysates.
      Western Blot Analysis: A 1:500 dilution of this antibody was used to detect Histone H3 in untreated and TSA-treated NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - 2022632 2022632
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - 2146031 2146031
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - 2428497 2428497
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - 2211063 2211063
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - 2262732 2262732
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - 2326272 2326272
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - JBC1771178 JBC1771178
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - NG1628015 NG1628015
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - NG1703398 NG1703398
      Anti-acetyl-Histone H3 (Lys9), clone Y28, Rabbit Monoclonal - NG1726966 NG1726966

      References

      Reference overviewApplicationPub Med ID
      Three-dimensional collagen I promotes gemcitabine resistance in vitro in pancreatic cancer cells through HMGA2-dependent histone acetyltransferase expression.
      Dangi-Garimella, S; Sahai, V; Ebine, K; Kumar, K; Munshi, HG
      PloS one  8  e64566  2013

      Show Abstract
      23696899 23696899
      Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.
      Ma, P; Pan, H; Montgomery, RL; Olson, EN; Schultz, RM
      Proceedings of the National Academy of Sciences of the United States of America  109  E481-9  2012

      Show Abstract
      Immunofluorescence22223663 22223663
      The action mechanism of the Myc inhibitor termed Omomyc may give clues on how to target Myc for cancer therapy.
      Savino, M; Annibali, D; Carucci, N; Favuzzi, E; Cole, MD; Evan, GI; Soucek, L; Nasi, S
      PloS one  6  e22284  2011

      Show Abstract
      21811581 21811581

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      Life Science Research > Antibodies and Assays > Primary Antibodies