06-598 | Anti-acetyl-Histone H4 Antibody

06-598
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      T, HICC, IP, WB, ChIPRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-598
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H4 Antibody
      Alternate Names
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatPurified
      Control
      • TSA-treated NIH/3T3, A431, L929 (mouse) and C6 (rat) cell extracts, human breast carcinoma.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-acetyl-Histone H4 Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a & has been validated in ChIP, ICC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation:
      5 μg of a previous lot immunoprecipitated acetylated histone H4 from 500 μg of acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours.

      Immunocytochemistry:
      5-10 μg/mL of a previous lot lot gave positive nuclear immunostaining in human A431 cells treated with sodium butyrate and fixed with ice-cold 1% paraformaldehyde in PBS.

      Chromatin Immunoprecipitation:
      This antibody has been reported by an independent laboratory to immunoprecipitate acetylated histone H4 from chromatin.
      Biological Information
      ImmunogenKLH-conjugated peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 (AGG(Ac)KGG(Ac)KGMG(Ac)KVGA(Ac)KRHS-C), acetylated on lysines 5,8,12 and 16.
      Epitopea.a. 2-19
      Concentration1 mg/mL
      HostRabbit
      SpecificityRecognizes acetylated histone H4.
      Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
      IsotypeIgG
      Species Reactivity
      • Tetrahymena
      • Human
      Species Reactivity NoteHuman and Tetrahymena. Other species not tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq]
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on acid-extracted proteins from HeLa cells treated with sodium butyrate.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected acetylated histone H4 in acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate. A previous lot detected acetylated histone H4 in Tetrahymena macronuclei. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
      Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H4 2474893
      Anti-acetyl-Histone H4 - 2387828 2387828
      Anti-acetyl-Histone H4 - 2424765 2424765
      Anti-acetyl-Histone H4 - 15793 15793
      Anti-acetyl-Histone H4 - 16728 16728
      Anti-acetyl-Histone H4 - 17942 17942
      Anti-acetyl-Histone H4 - 19602 19602
      Anti-acetyl-Histone H4 - 2019720 2019720
      Anti-acetyl-Histone H4 - 2302181 2302181
      Anti-acetyl-Histone H4 - 23224 23224

      References

      Reference overviewApplicationSpeciesPub Med ID
      Hepatic TRAP80 selectively regulates lipogenic activity of liver X receptor.
      Kim, GH; Oh, GS; Yoon, J; Lee, GG; Lee, KU; Kim, SW
      The Journal of clinical investigation  125  183-93  2015

      Show Abstract
      25437875 25437875
      Synergistic antitumor interactions between MK-1775 and panobinostat in preclinical models of pancreatic cancer.
      Wang, G; Niu, X; Zhang, W; Caldwell, JT; Edwards, H; Chen, W; Taub, JW; Zhao, L; Ge, Y
      Cancer letters  356  656-68  2015

      Show Abstract
      Western BlottingHuman25458954 25458954
      Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area.
      Koo, JW; Mazei-Robison, MS; LaPlant, Q; Egervari, G; Braunscheidel, KM; Adank, DN; Ferguson, D; Feng, J; Sun, H; Scobie, KN; Damez-Werno, DM; Ribeiro, E; Peña, CJ; Walker, D; Bagot, RC; Cahill, ME; Anderson, SA; Labonté, B; Hodes, GE; Browne, H; Chadwick, B; Robison, AJ; Vialou, VF; Dias, C; Lorsch, Z; Mouzon, E; Lobo, MK; Dietz, DM; Russo, SJ; Neve, RL; Hurd, YL; Nestler, EJ
      Nature neuroscience  18  415-22  2015

      Show Abstract
      25643298 25643298
      ZEB1-associated drug resistance in cancer cells is reversed by the class I HDAC inhibitor mocetinostat.
      Meidhof, S; Brabletz, S; Lehmann, W; Preca, BT; Mock, K; Ruh, M; Schüler, J; Berthold, M; Weber, A; Burk, U; Lübbert, M; Puhr, M; Culig, Z; Wellner, U; Keck, T; Bronsert, P; Küsters, S; Hopt, UT; Stemmler, MP; Brabletz, T
      EMBO molecular medicine  7  831-47  2015

      Show Abstract
      25872941 25872941
      Compensatory induction of MYC expression by sustained CDK9 inhibition via a BRD4-dependent mechanism.
      Lu, H; Xue, Y; Xue, Y; Yu, GK; Arias, C; Lin, J; Fong, S; Faure, M; Weisburd, B; Ji, X; Mercier, A; Sutton, J; Luo, K; Gao, Z; Zhou, Q
      eLife  4  e06535  2015

      Show Abstract
      26083714 26083714
      Berberine reverses abnormal expression of L-type pyruvate kinase by DNA demethylation and histone acetylation in the livers of the non-alcoholic fatty disease rat.
      Zhang, Y; Chang, X; Song, X; Chen, C; Chen, H; Lu, Z; Gao, X; Lu, D
      International journal of clinical and experimental medicine  8  7535-43  2015

      Show Abstract
      26221297 26221297
      5-Hydroxymethylcytosine Plays a Critical Role in Glioblastomagenesis by Recruiting the CHTOP-Methylosome Complex.
      Takai, H; Masuda, K; Sato, T; Sakaguchi, Y; Suzuki, T; Suzuki, T; Koyama-Nasu, R; Nasu-Nishimura, Y; Katou, Y; Ogawa, H; Morishita, Y; Kozuka-Hata, H; Oyama, M; Todo, T; Ino, Y; Mukasa, A; Saito, N; Toyoshima, C; Shirahige, K; Akiyama, T
      Cell reports  9  48-60  2014

      Show Abstract
      25284789 25284789
      Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement.
      Paino, F; La Noce, M; Tirino, V; Naddeo, P; Desiderio, V; Pirozzi, G; De Rosa, A; Laino, L; Altucci, L; Papaccio, G
      Stem cells (Dayton, Ohio)  32  279-89  2014

      Show Abstract
      Western Blotting24105979 24105979
      Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans.
      Lee, CC; Tsai, YT; Kao, CW; Lee, LW; Lai, HJ; Ma, TH; Chang, YS; Yeh, NH; Lo, SJ
      Cell death & disease  5  e1158  2014

      Show Abstract
      24722283 24722283
      Cooperation between BRCA1 and vitamin D is critical for histone acetylation of the p21waf1 promoter and growth inhibition of breast cancer cells and cancer stem-like cells.
      Pickholtz, I; Saadyan, S; Keshet, GI; Wang, VS; Cohen, R; Bouwman, P; Jonkers, J; Byers, SW; Papa, MZ; Yarden, RI
      Oncotarget  5  11827-46  2014

      Show Abstract
      25460500 25460500