06-866 | Anti-acetyl-Histone H4 Antibody

06-866
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, T, EuWB, ICC, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-866
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-acetyl-Histone H4 Antibody
      Alternate Names
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatSerum
      Control
      • TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.
      PresentationWhole antiserum containing 0.05% sodium azide.
      Applications
      ApplicationUse Anti-acetyl-Histone H4 Antibody (Rabbit Polyclonal Antibody) has been published and validated in ChIP, WB, ICC to detect acetyl-Histone H4 also known as H4 histone family member A, histone 1-H4a.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
      Epitopea.a. 2-19
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAcetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
      IsotypeIgG
      Species Reactivity
      • Human
      • Tetrahymena
      • Eukaryote
      Species Reactivity NoteHuman and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • IST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • 4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

      Western Blot Analysis:
      1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H4 (rabbit antiserum) - DAM1427455 DAM1427455
      Anti-acetyl-Histone H4 - ANTI-ACETYL-HISTONE
      Anti-acetyl-Histone H4 - 2384725 2384725
      Anti-acetyl-Histone H4 - 2459612 2459612
      Anti-acetyl-Histone H4 - 17428 17428
      Anti-acetyl-Histone H4 - 18784 18784
      Anti-acetyl-Histone H4 - 19336 19336
      Anti-acetyl-Histone H4 - 2019734 2019734
      Anti-acetyl-Histone H4 - 20667 20667
      Anti-acetyl-Histone H4 - 2073114 2073114

      References

      Reference overviewApplicationSpeciesPub Med ID
      Transcriptional regulation of the human TNFSF11 gene in T cells via a cell type-selective set of distal enhancers.
      Bishop, KA; Wang, X; Coy, HM; Meyer, MB; Gumperz, JE; Pike, JW
      Journal of cellular biochemistry  116  320-30  2015

      Show Abstract
      25211367 25211367
      Mechanistic analysis of the role of bromodomain-containing protein 4 (BRD4) in BRD4-NUT oncoprotein-induced transcriptional activation.
      Wang, R; You, J
      The Journal of biological chemistry  290  2744-58  2015

      Show Abstract
      Immunofluorescence25512383 25512383
      Loss of the Notch effector RBPJ promotes tumorigenesis.
      Kulic, I; Robertson, G; Chang, L; Baker, JH; Lockwood, WW; Mok, W; Fuller, M; Fournier, M; Wong, N; Chou, V; Robinson, MD; Chun, HJ; Gilks, B; Kempkes, B; Thomson, TA; Hirst, M; Minchinton, AI; Lam, WL; Jones, S; Marra, M; Karsan, A
      The Journal of experimental medicine  212  37-52  2015

      Show Abstract
      25512468 25512468
      Evaluation of the synuclein-γ (SNCG) gene as a PPARγ target in murine adipocytes, dorsal root ganglia somatosensory neurons, and human adipose tissue.
      Dunn, TN; Akiyama, T; Lee, HW; Kim, JB; Knotts, TA; Smith, SR; Sears, DD; Carstens, E; Adams, SH
      PloS one  10  e0115830  2015

      Show Abstract
      25756178 25756178
      Deacetylase inhibitors repress STAT5-mediated transcription by interfering with bromodomain and extra-terminal (BET) protein function.
      Pinz, S; Unser, S; Buob, D; Fischer, P; Jobst, B; Rascle, A
      Nucleic acids research  43  3524-45  2015

      Show Abstract
      25769527 25769527
      HDAC8, A Potential Therapeutic Target for the Treatment of Malignant Peripheral Nerve Sheath Tumors (MPNST).
      Lopez, G; Bill, KL; Bid, HK; Braggio, D; Constantino, D; Prudner, B; Zewdu, A; Batte, K; Lev, D; Pollock, RE
      PloS one  10  e0133302  2015

      Show Abstract
      26200462 26200462
      Conserved Epigenetic Mechanisms Could Play a Key Role in Regulation of Photosynthesis and Development-Related Genes during Needle Development of Pinus radiata.
      Valledor, L; Pascual, J; Meijón, M; Escandón, M; Cañal, MJ
      PloS one  10  e0126405  2015

      Show Abstract
      25965766 25965766
      A vlincRNA participates in senescence maintenance by relieving H2AZ-mediated repression at the INK4 locus.
      Lazorthes, S; Vallot, C; Briois, S; Aguirrebengoa, M; Thuret, JY; St Laurent, G; Rougeulle, C; Kapranov, P; Mann, C; Trouche, D; Nicolas, E
      Nature communications  6  5971  2015

      Show Abstract
      25601475 25601475
      Canine spontaneous head and neck squamous cell carcinomas represent their human counterparts at the molecular level.
      Liu, D; Xiong, H; Ellis, AE; Northrup, NC; Dobbin, KK; Shin, DM; Zhao, S
      PLoS genetics  11  e1005277  2015

      Show Abstract
      26030765 26030765
      Class I histone deacetylase inhibitors inhibit the retention of BRCA1 and 53BP1 at the site of DNA damage.
      Fukuda, T; Wu, W; Okada, M; Maeda, I; Kojima, Y; Hayami, R; Miyoshi, Y; Tsugawa, K; Ohta, T
      Cancer science  106  1050-6  2015

      Show Abstract
      26053117 26053117

      Posters

      Title
      Histone Modifications

      FAQ

      QuestionAnswer
      How was this antibody made? What modifications will it recognize?The Anti-acetyl-Histone H4 was raised against the tetra acetylated H4. This antibody will recognize intermediately acetylated H4, but we are unsure what the reactivity for the antibody is against tetra-acetylated H4. We know it will not recognize acetylation on lysine 16, however it does recognize acetylation on the other three sites. A protein does not require acetylation on all four sites for this antibody to recognize it.