06-598-AF488 | Anti-acetyl-Histone H4 Antibody, AlexaFluor® 488 Conjugate

06-598-AF488
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue Number06-598-AF488
      DescriptionAnti-acetyl-Histone H4 Antibody, AlexaFluor® 488 Conjugate
      Alternate Names
      • Histone H4, major
      • acetyl-Histone H4, AlexaFluor® 488 Conjugate
      • Histone H4, minor
      • Histone H4
      Background InformationHistone H4 (UniProt P02310; P69151; P69152) is encoded by the HHF1 (ORF TTHERM_00498190) and HHF2 (ORF TTHERM_00189170) gene (Gene ID 7823613) in protozoa Tetrahymena. Histone H4 is one of the main histone proteins involved in the structure of chromatin in both prokaryotic and eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
      Applications
      ApplicationThis Anti-acetyl-Histone H4 Antibody, AlexaFluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of acetyl-Histone H4.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected acetyl-Histone H4 in HeLa, A431, and NIH/3T3 cells.
      The unconjugated antibody (Cat. No. 06-598) is shown to be suitable also for Immunoprecipitation (IP), Chromatin IP (ChIP), and Western blotting applications.
      Biological Information
      ImmunogenKLH-conjugated peptide corresponding to amino acids 2-19 of Tetrahymena histone H4 (AGG(Ac)KGG(Ac)KGMG(Ac)KVGA(Ac)KRHS-C), acetylated on lysines 5,8,12 and 16.
      Epitopea.a. 2-19
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes acetylated histone H4. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells. May crossreact with other acetylated proteins.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Tetrahymena, Protozoa based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HHF1
      • HHF2
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~11 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HUVEC cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected acetyl-Histone H4 in HUVECs.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications