06-1352-I | Anti-acetyl Histone H4 (Lys12) Antibody

06-1352-I
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, DBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1352-I
      DescriptionAnti-acetyl Histone H4 (Lys12) Antibody
      Alternate Names
      • Histone H4
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-acetyl Histone H4 (Lys12) Antibody is validated for use in western blotting, ICC & dot blot for the detection of acetyl Histone H4 (Lys12).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Dot Blot
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected acetyl Histone H4 (Lys12) in HeLa cells.

      Dot Blot (Specificity) Analysis: 0.1 µg/mL from a representative lot detected acetyl Histone H4 (Lys12) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and in an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 acetylated at Lys12.
      EpitopeN-terminus
      Concentration0.5 mg/mL
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H4A
      • H4/A
      • H4FA
      • HIST1H4B
      • H4/I
      • H4FI
      • HIST1H4C
      • H4/G
      • H4FG
      • HIST1H4D
      • H4/B
      • H4FB
      • HIST1H4E
      • H4/J
      • H4FJ
      • HIST1H4F
      • H4/C
      • H4FC
      • HIST1H4H
      • H4/H
      • H4FH
      • HIST1H4I
      • H4/M
      • H4FM
      • HIST1H4J
      • H4/E
      • H4FE
      • HIST1H4K
      • H4/D
      • H4FD
      • HIST1H4L
      • H4/K
      • H4FK
      • HIST2H4A
      • H4/N
      • H4F2
      • H4FN
      • HIST2H4
      • HIST2H4B
      • H4/O
      • H4FO
      • HIST4H4
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~13 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in sodium butyrate treated HeLa acid extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl Histone H4 (Lys12) in 10 µg of sodium butyrate treated HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications