05-1128 | Anti-active-EGFR (intracellular) Antibody, clone 74

100 µg  
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      Replacement Information
      Replacement Information04-338 is the recommended replacement for Discontinued catalog number 05-1128

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ELISA, IP, IH(P), ICCMPurifiedMonoclonal Antibody
      Catalogue Number05-1128
      DescriptionAnti-active-EGFR (intracellular) Antibody, clone 74
      Alternate Names
      • Receptor tyrosine-protein kinase ErbB-1
      Background InformationEGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. EGFR is found in most solid tumors and it is known to be essential for mediation of both proliferative and survival signals to cells. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream including the classical MAPK signaling pathway, that leads to DNA synthesis, cell proliferation, adhesion, and migration.

      Product Information
      • EGF stimulated A431 cell lysate.
      PresentationPurified mouse monoclonal IgG1 in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, 15 mg/mL BSA, 50% glycerol, with 0.1% sodium azide.
      ApplicationThis Anti-active-EGFR (intracellular) Antibody, clone 74 is validated for use in WB, ELISA, IP, IH(P), IC for the detection of active-EGFR (intracellular).
      Key Applications
      • Western Blotting
      • ELISA
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunoprecipitation: A previous lot of this antibody was used to detect native and denatured EGFR.

      ELISA: A previous lot of this antibody was used in ELISA.

      Immunocytochemistry: A previous lot of this antibody was used in IC.
      Optimal working dilutions must be determined by end user.

      Immunohistochemistry(paraffin): Representative testing from a previous lot.
      Biological Information
      ImmunogenTyrosine-phosphorylated epidermal growth factor receptor.
      Epitopeintracellular, activated EGFR
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts strongly and specifically with the intracellular domain of the activated and phosphorylated EGFR in human cell lines and does not react with other phosphorylated proteins.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman. Expected to react with mouse and rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      • HER1
      • PIG61
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin.
      FUNCTION: Isoform 2/truncated isoform may act as an antagonist.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C-terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. Binds MUC1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
      SUBCELLULAR LOCATION: Isoform 2: Secreted.
      ALTERNATIVE PRODUCTS: 4 named isoforms [FASTA] produced by alternative splicing.
      Molecular Weight170 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on EGF treated A431 lysates.
      Western Blot Analysis: 1:500 dilution of this lot detected VEGFR on 10 μg of EGF treated A431 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 1 year from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information

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