CBL440 | Anti-c-Fos Antibody

CBL440
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, ICCShPurifiedPolyclonal Antibody
      Description
      Catalogue NumberCBL440
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-c-Fos Antibody
      Alternate Names
      • G0/G1 Switch Regulatory Protein 7
      References
      Product Information
      FormatPurified
      PresentationThe purified immunoglobulin is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-c-Fos Antibody is a Sheep Polyclonal Antibody for detection of c-Fos also known as G0/G1 Switch Regulatory Protein 7 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunoblotting: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. CBL 440 detects a band at 55-60 kDa on immunoblots of stimulated HeLa and HL-60 lysates.

      Immunocytochemistry: CBL 440 has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (where CBL 440 detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala-Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.
      HostSheep
      Specificityc-fos is a 55 kDa nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures.

      KNOWN SPECIES CROSS REACTIVITY: Known to cross-react with rat
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
      Gene Symbol
      • FOS
      • G0S7
      • c-fos
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01100 # Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
      SIZE: 380 amino acids; 40695 Da
      SUBUNIT: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: P01100 ## Belongs to the bZIP family. Fos subfamily. & Contains 1 bZIP domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      SHEEP ANTI- C-FOS ONCOPROTEINS -2515373 2515373
      SHEEP ANTI- C-FOS ONCOPROTEINS -2795675 2795675
      SHEEP ANTI- C-FOS ONCOPROTEINS POLYCLONAL ANTIBODY 2886498
      SHEEP ANTI- C-FOS ONCOPROTEINS POLYCLONAL ANTIBODY - 2054781 2054781
      SHEEP ANTI- C-FOS ONCOPROTEINS POLYCLONAL ANTIBODY - 2293674 2293674

      References

      Reference overviewPub Med ID
      Transcription activation by serum, PDGF, and TPA through the c-fos DSE: cell type specific requirements for induction.
      Siegfried, Z and Ziff, E B
      Oncogene, 4: 3-11 (1989)  1989

      Show Abstract
      2915902 2915902
      Long-lasting and sequential increase of c-fos oncoprotein expression in kainic acid-induced status epilepticus.
      Le Gal La Salle, G
      Neurosci. Lett., 88: 127-30 (1988)  1988

      Show Abstract
      3132654 3132654
      Effects of epidermal growth factor and platelet-derived growth factor on c-fos and c-myc mRNA levels in normal human fibroblasts.
      Paulsson, Y, et al.
      Exp. Cell Res., 171: 186-94 (1987)  1987

      Show Abstract
      3497813 3497813
      Dissociation of c-fos induction from macrophage differentiation in human myeloid leukemic cell lines.
      Calabretta, B
      Mol. Cell. Biol., 7: 769-74 (1987)  1987

      3547082 3547082
      Induction of c-fos-like protein in spinal cord neurons following sensory stimulation.
      Hunt, S P, et al.
      Nature, 328: 632-4  1987

      3112583 3112583
      Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.
      van Straaten, F, et al.
      Proc. Natl. Acad. Sci. U.S.A., 80: 3183-7 (1983)  1983

      Show Abstract
      6574479 6574479

      Data Sheet

      Title
      SHEEP ANTI- C-FOS ONCOPROTEINS POLYCLONAL ANTIBODY - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies