09-754 | Anti-c-Jun Antibody

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, Ch, CaWBRbPurifiedPolyclonal Antibody
      Catalogue Number09-754
      DescriptionAnti-c-Jun Antibody
      Alternate Names
      • JNK1 alpha protein kinase
      • JNK1 beta protein kinase
      • JUN N-terminal kinase
      • Stress-activated protein kinase JNK1
      • c-Jun N-terminal kinase 1
      • mitogen-activated protein kinase 8
      • mitogen-activated protein kinase 8 isoform JNK1 alpha1
      • mitogen-activated protein kinase 8 isoform JNK1 beta2
      • protein kinase JNK1
      Background Informationc-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced
      signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
      Product Information
      • NIH/3T3 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      ApplicationAnti-c-Jun Antibody is a Rabbit Polyclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the internal domain of c-Jun.
      EpitopeInternal Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes the internal domain of c-Jun.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Chicken
      • Canine
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with rat, cow, chicken, and dog based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC
      • JNK
      • JNK-46
      • JNK1
      • JNK1A2
      • JNK21B1/2
      • OTTHUMP00000019552
      • OTTHUMP00000019555
      • OTTHUMP00000019556
      • OTTHUMP00000019558
      • PRKM8
      • SAPK1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4).

      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      COFACTOR: Magnesium.

      ENZYME REGULATIION: Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.

      SUBUNIT STRUCTURE: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and RGNEF By similarity. Interacts with NFATC4.

      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

      PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

      Contains 1 protein kinase domain.

      Molecular Weight~40-42 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected c-Jun in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of recipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-c-Jun - 2118997 2118997
      Anti-c-Jun - 2145094 2145094
      Anti-c-Jun - 2147097 2147097
      Anti-c-Jun - 2440182 2440182
      Anti-c-Jun - 2459511 2459511
      Anti-c-Jun - 2211075 2211075
      Anti-c-Jun - NG1622163 NG1622163
      Anti-c-Jun - NG1878844 NG1878844


      Reference overviewApplicationPub Med ID
      Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model.
      Yao, X; Carlson, D; Sun, Y; Ma, L; Wolf, SE; Minei, JP; Zang, QS
      PloS one  10  e0139416  2015

      Show Abstract
      Western Blotting26448624 26448624
      The detergent-soluble cytoplasmic pool of survivin suppresses anoikis and its expression is associated with metastatic disease of human colon cancer.
      Hori, M; Miki, T; Okamoto, M; Yazama, F; Konishi, H; Kaneko, H; Shimamoto, F; Ota, T; Temme, A; Tatsuka, M
      PloS one  8  e55710  2013

      Show Abstract
      Western Blotting23405201 23405201

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      Life Science Research > Antibodies and Assays > Primary Antibodies