05-1076 | Anti-c-Jun Antibody, clone 6E4.4

05-1076
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1076
      Replaces04-210
      DescriptionAnti-c-Jun Antibody, clone 6E4.4
      Alternate Names
      • JNK1 alpha protein kinase
      • JNK1 beta protein kinase
      • JUN N-terminal kinase
      • Stress-activated protein kinase JNK1
      • c-Jun N-terminal kinase 1
      • mitogen-activated protein kinase 8
      • mitogen-activated protein kinase 8 isoform JNK1 alpha1
      • mitogen-activated protein kinase 8 isoform JNK1 beta2
      • protein kinase JNK1
      Background Informationc-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).

      Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to c-Jun.
      Clone6E4.4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects c-Jun, regardless of phosphorylation state.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involvedin both translocations and deletions in human malignancies.
      Gene Symbol
      • EC 2.7.11.24
      • JNK
      • JNK-46
      • JNK1
      • JNK1A2
      • JNK21B1/2
      • OTTHUMP00000019552
      • OTTHUMP00000019555
      • OTTHUMP00000019556
      • OTTHUMP00000019558
      • PRKM8
      • SAPK1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4).

      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      COFACTOR: Magnesium.

      ENZYME REGULATION: Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.

      SUBUNIT STRUCTURE: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and RGNEF By similarity. Interacts with NFATC4.

      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

      PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

      Contains 1 protein kinase domain.

      Molecular Weight~36-43 kDa.
      Multiple bands may be visible, indicating various phosphorylation states.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

      Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-c-Jun, clone 6E4.4 (mouse monoclonal) Monoclonal Antibody Q2922325
      Anti-c-Jun, clone 6E4.4 - 2148277 2148277
      Anti-c-Jun, clone 6E4.4 - 2395703 2395703
      Anti-c-Jun, clone 6E4.4 - 2446884 2446884
      Anti-c-Jun, clone 6E4.4 - 2196051 2196051
      Anti-c-Jun, clone 6E4.4 - 2211967 2211967
      Anti-c-Jun, clone 6E4.4 - 2283380 2283380
      Anti-c-Jun, clone 6E4.4 - NG1624969 NG1624969
      Anti-c-Jun, clone 6E4.4 - NG1811492 NG1811492
      Anti-c-Jun, clone 6E4.4 - NG1887093 NG1887093

      References

      Reference overviewPub Med ID
      Milk-derived tripeptides IPP (Ile-Pro-Pro) and VPP (Val-Pro-Pro) promote adipocyte differentiation and inhibit inflammation in 3T3-F442A cells.
      Chakrabarti, S; Wu, J
      PloS one  10  e0117492  2015

      Show Abstract
      25714093 25714093
      G-protein coupled receptor 30 (GPR30): a novel regulator of endothelial inflammation.
      Chakrabarti, S; Davidge, ST
      PloS one  7  e52357  2012

      Show Abstract
      23285008 23285008

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      06-748 Anti-JNK/SAPK1 Antibody Show Pricing & Availability
      07-175 Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody Show Pricing & Availability
      09-754 Anti-c-Jun Antibody Show Pricing & Availability
      FCMAB122F Milli-Mark™ Anti-c-Jun Antibody, clone 6E4, FITC Conjugate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies