CBL434 | Anti-c-myc Antibody, clone 3C7

CBL434
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL434
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-c-myc Antibody, clone 3C7
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationDetect c-myc also known as Proto-oncogene c-Myc with Anti-c-myc Antibody, clone 3C7 (Mouse Monoclonal Antibody), that has been demonstrated to work in ELISA, IP & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blotting

      Immunoprecipitation

      Immunoassay detection of c-myc

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide: Cys-Ser-Thr-Ser-Ser-Leu-Tyr-Leu-Gln-Asp-Leu-Ser-Ala-Ala-Ala-Ser-Glu-Cys selected from the human c-myc sequence (amino acids (171-188). The peptide was conjugated to keyhole limpet haemocyanin.
      Epitopea.a. 171-188
      Clone3C7
      HostMouse
      SpecificityIn immunoblotting this antibody detects one major band at 62-64 kDa which can be blocked by pre-incubation with the immunizing peptide. In immunoprecipitation the antibody detects a 62 kDa band from cell lysates.

      KNOWN SPECIES CROSS REACTIVITY: This antibody is known to cross react with mouse c-myc
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN C-MYC -2703703 2703703
      MOUSE ANTI-HUMAN C-MYC -2841953 2841953
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY 2955317
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY - 2141810 2141810
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY - 2385939 2385939
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY - 2427474 2427474
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY - 2053928 2053928
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY - 2199598 2199598
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY - 2293711 2293711
      MOUSE ANTI-HUMAN C-MYC MONOCLONAL ANTIBODY -2530089 2530089

      References

      Reference overviewPub Med ID
      MAFIP is a tumor suppressor in cervical cancer that inhibits activation of the nuclear factor-kappa B pathway.
      Yang Li,Yao Yu,Yan Zhang,Yufei Zhou,Chao Li,Jianbing Zhu,Hanying Yuan,Hong Lu
      Cancer science  102  2011

      Show Abstract
      21834855 21834855
      microRNA-141 is involved in a nasopharyngeal carcinoma-related genes network.
      Zhang L, Deng T, Li X, Liu H, Zhou H, Ma J, Wu M, Zhou M, Shen S, Li X, Niu Z, Zhang W, Shi L, Xiang B, Lu J, Wang L, Li D, Tang H, Li G
      Carcinogenesis  31  559-66 Epub 2010 Jan 6  2010

      Show Abstract
      20053927 20053927
      A sensitive and quantitative enzyme-linked immunosorbence assay for the c-myc and N-myc oncoproteins.
      Moore, J P, et al.
      Oncogene Res., 2: 65-80 (1987)  1987

      Show Abstract
      3333275 3333275
      Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
      Evan, G I, et al.
      Mol. Cell. Biol., 5: 3610-6 (1985)  1985

      Show Abstract
      3915782 3915782

      Data Sheet

      Title
      Anti-c-myc, a.a. 171-188, clone 3C7 - Data Sheet

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