05-808 | Anti-dimethyl-Histone H3 (Arg2) Antibody, rabbit monoclonal

05-808
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HDB, WB, PIA, MplexRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-808
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Arg2) Antibody, rabbit monoclonal
      Alternate Names
      • H3R2me2
      • Histone H3 (di methyl R2)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      Control
      • Acid extracts from HeLa cells
      Presentation0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 100mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%
      Applications
      ApplicationUse Anti-dimethyl-Histone H3 (Arg2) Antibody (Rabbit Monoclonal Antibody) validated in DB, WB, PIA, Mplex to detect dimethyl-Histone H3 (Arg2) also known as H3R2me2, Histone H3 (di methyl R2).
      Key Applications
      • Dot Blot
      • Western Blotting
      • Peptide Inhibition Assay
      • Multiplexing
      Biological Information
      Immunogenpeptide containing the sequence AdimRTKQ, corresponding to dimethyl-arginine 2 of human histone H3.
      HostRabbit
      Specificitydimethyl-arginine at residue 2 of histone H3
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Methylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in acid-extracted proteins from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Arg2) - 2433350 2433350
      Anti-dimethyl-Histone H3 (Arg2) - 2277851 2277851
      Anti-dimethyl-Histone H3 (Arg2) - 26925 26925
      Anti-dimethyl-Histone H3 (Arg2) - DAM1805239 DAM1805239

      References

      Reference overviewApplicationPub Med ID
      A gain-of-function mouse model identifies PRMT6 as a NF-κB coactivator.
      Di Lorenzo, A; Yang, Y; Macaluso, M; Bedford, MT
      Nucleic acids research  42  8297-309  2014

      Show Abstract
      24939901 24939901
      Early patterning of cloned mouse embryos contributes to post-implantation development.
      Zichuan Liu,Tang Hai,Xiangpeng Dai,Xiaoyang Zhao,Yingying Wang,Vincent Brochard,Shuya Zhou,Haifeng Wan,Haijiang Zhang,Liu Wang,Qi Zhou,Nathalie Beaujean
      Developmental biology  368  2012

      Show Abstract
      22659081 22659081
      Characterization of the PRMT gene family in rice reveals conservation of arginine methylation.
      Ahmad, A; Dong, Y; Cao, X
      PloS one  6  e22664  2011

      Show Abstract Full Text Article
      Western Blotting21853042 21853042
      Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
      Shechter, D; Nicklay, JJ; Chitta, RK; Shabanowitz, J; Hunt, DF; Allis, CD
      The Journal of biological chemistry  284  1064-74  2009

      Show Abstract
      18957438 18957438

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      Life Science Research > Antibodies and Assays > Primary Antibodies