07-274 Anti-dimethyl-Histone H3 (Lys36) Antibody

200 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Ch, Dr, Vrt, Yeast (S. cerevisiae)ChIP, WB, DBRbSerumPolyclonal Antibody
      Catalogue Number07-274
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Lys36) Antibody
      Alternate Names
      • H3K36me2
      • Histone H3 (di methyl K36)
      • H3 histone family, member T
      • histone 3
      • H3
      • histone cluster 3
      • H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      Product Information
      • Sodium butyrate treated, acid extracted HeLa cell lysate.
      PresentationRabbit polyclonal IgG immunodepleted antiserum in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC. Serum was passed over immunoaffinity column containing the non-modified peptide.
      ApplicationUse Anti-dimethyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect dimethyl-Histone H3 (Lys36) also known as H3K36me2, Histone H3 (di methyl K36).
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • Dot Blot
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 30-38 (CPATGGV(dimethyl-K)KP) of Histone H3 with a N-terminal C added for conjugation. The immunizing sequence is conserved from Tetrahymena to human.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes methylated Histone H3 (Lys36), Mr 17 kDa as determined by Immunoblotting. Does not cross react with peptides containing dimethyl-histone H4 Lys20, nor Histone H3 Lys4, Lys9, or Lys27.
      Species Reactivity
      • Mouse
      • Human
      • Chicken
      • Drosophila
      • Vertebrates
      • Yeast (S. cerevisiae)
      Species Reactivity NoteHuman, mouse, chicken, Drosophilia, and fission yeast. A broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      • Methylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on in acid-extracted proteins from sodium butyrate treated cells.

      Western Blot Analysis:
      A 1:1000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from untreated HeLa cells (Catalog # 13-112). The antibody did not detect recombinant Xenopus laevis histone H3 purified from E. coli (Catalog # 14-411).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Lys36)2470872
      Anti-dimethyl-Histone H3 (Lys36) - 23939012393901
      Anti-dimethyl-Histone H3 (Lys36) - 07020518160702051816
      Anti-dimethyl-Histone H3 (Lys36) - 20197352019735
      Anti-dimethyl-Histone H3 (Lys36) - 22009312200931
      Anti-dimethyl-Histone H3 (Lys36) - 2212222122
      Anti-dimethyl-Histone H3 (Lys36) - 22778582277858
      Anti-dimethyl-Histone H3 (Lys36) - 29312302931230
      Anti-dimethyl-Histone H3 (Lys36) - 3155331553
      Anti-dimethyl-Histone H3 (Lys36) - 31694943169494


      Reference overviewApplicationSpeciesPub Med ID
      Impaired p32 regulation caused by the lymphoma-prone RECQ4 mutation drives mitochondrial dysfunction.
      Wang, JT; Xu, X; Alontaga, AY; Chen, Y; Liu, Y
      Cell reports  7  848-58  2014

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      24746816 24746816
      Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation.
      Alvarez-Saavedra, M; De Repentigny, Y; Lagali, PS; Raghu Ram, EV; Yan, K; Hashem, E; Ivanochko, D; Huh, MS; Yang, D; Mears, AJ; Todd, MA; Corcoran, CP; Bassett, EA; Tokarew, NJ; Kokavec, J; Majumder, R; Ioshikhes, I; Wallace, VA; Kothary, R; Meshorer, E; Stopka, T; Skoultchi, AI; Picketts, DJ
      Nature communications  5  4181  2014

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      New insights into somatic embryogenesis: leafy cotyledon1, baby boom1 and WUSCHEL-related homeobox4 are epigenetically regulated in Coffea canephora.
      Nic-Can, GI; López-Torres, A; Barredo-Pool, F; Wrobel, K; Loyola-Vargas, VM; Rojas-Herrera, R; De-la-Peña, C
      PloS one  8  e72160  2013

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      The chromatin remodeling and mRNA splicing functions of the Brahma (SWI/SNF) complex are mediated by the SNR1/SNF5 regulatory subunit.
      Zraly, CB; Dingwall, AK
      Nucleic acids research  40  5975-87  2012

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      KDM3B is the H3K9 demethylase involved in transcriptional activation of lmo2 in leukemia.
      Kim, JY; Kim, KB; Eom, GH; Choe, N; Kee, HJ; Son, HJ; Oh, ST; Kim, DW; Pak, JH; Baek, HJ; Kook, H; Hahn, Y; Kook, H; Chakravarti, D; Seo, SB
      Molecular and cellular biology  32  2917-33  2012

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      22615488 22615488
      The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology, and the metabolome of fibroblasts.
      Janzer, A; Stamm, K; Becker, A; Zimmer, A; Buettner, R; Kirfel, J
      The Journal of biological chemistry  287  30984-92  2012

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      22825849 22825849
      JMJD5, a Jumonji C (JmjC) domain-containing protein, negatively regulates osteoclastogenesis by facilitating NFATc1 protein degradation.
      Youn, MY; Yokoyama, A; Fujiyama-Nakamura, S; Ohtake, F; Minehata, K; Yasuda, H; Suzuki, T; Kato, S; Imai, Y
      The Journal of biological chemistry  287  12994-3004  2012

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      22375008 22375008
      KNOX1 is expressed and epigenetically regulated during in vitro conditions in Agave spp.
      De-la-Peña, C; Nic-Can, G; Ojeda, G; Herrera-Herrera, JL; López-Torres, A; Wrobel, K; Robert-Díaz, ML
      BMC plant biology  12  203  2012

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      The histone demethylase Jhdm1a regulates hepatic gluconeogenesis.
      Pan, D; Mao, C; Zou, T; Yao, AY; Cooper, MP; Boyartchuk, V; Wang, YX
      PLoS genetics  8  e1002761  2012

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      22719268 22719268
      Arabidopsis HDA6 regulates locus-directed heterochromatin silencing in cooperation with MET1.
      To, TK; Kim, JM; Matsui, A; Kurihara, Y; Morosawa, T; Ishida, J; Tanaka, M; Endo, T; Kakutani, T; Toyoda, T; Kimura, H; Yokoyama, S; Shinozaki, K; Seki, M
      PLoS genetics  7  e1002055  2011

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