04-768 | Anti-dimethyl-Histone H3 (Lys9) Antibody, clone MC554, rabbit monoclonal

04-768
100 µL  
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      Overview

      Replacement Information
      Replacement InformationCat. # 05-768. 05-768 will only be availabe on a custom basis.

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, WB, PIA, DBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-768
      Replaces05-768
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H3 (Lys9) Antibody, clone MC554, rabbit monoclonal
      Alternate Names
      • H3K4me2
      • Histone H3 (di methyl K4)
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.

      The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Acid-extracted proteins from log phase HeLa cells
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationAnti-dimethyl-Histone H3 (Lys9) Antibody, clone MC554 is a rabbit monoclonal antibody for detection of dimethyl-Histone H3 (Lys9) also known as H3K4me2, Histone H3 (di methyl K4) & has been validated in IF, WB, PIA, DB.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Peptide Inhibition Assay
      • Dot Blot
      Biological Information
      ImmunogenPeptide containing dimethyl-lysine at residue 9 of human histone H3
      CloneMC554
      HostRabbit
      SpecificityHistone H3 dimethylated on lysine 9
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteBroad species cross reactivity is expected
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
      Gene Symbol
      • HIST3H3
      • H3FT
      • MGC126886
      • H3t
      • MGC126888
      • H3T
      • H3/g
      • H3.4
      • H3/t
      Modifications
      • Methylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid-extracted proteins from HeLa cells; the antibody did not detect unmethylated recombinant Histone H3 (Catalog #14-494)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H3 (Lys9), clone MC554 - 2431957 2431957
      Anti-dimethyl-Histone H3 (Lys9), -2548050 2548050
      Anti-dimethyl-Histone H3 (Lys9), -2562529 2562529
      Anti-dimethyl-Histone H3 (Lys9), -2658747 2658747
      Anti-dimethyl-Histone H3 (Lys9), clone MC554 - 2179365 2179365
      Anti-dimethyl-Histone H3 (Lys9), clone MC554 - DAM1498958 DAM1498958

      References

      Reference overviewApplicationPub Med ID
      Radiation-induced alterations of histone post-translational modification levels in lymphoblastoid cell lines.
      Maroschik, B; Gürtler, A; Krämer, A; Rößler, U; Gomolka, M; Hornhardt, S; Mörtl, S; Friedl, AA
      Radiation oncology (London, England)  9  15  2014

      Show Abstract
      Western Blotting24406105 24406105
      Suv39h1 mediates AP-2α-dependent inhibition of C/EBPα expression during adipogenesis.
      Zhang, ZC; Liu, Y; Li, SF; Guo, L; Zhao, Y; Qian, SW; Wen, B; Tang, QQ; Li, X
      Molecular and cellular biology  34  2330-8  2014

      Show Abstract
      Western Blotting24732798 24732798
      Histone acetylation accompanied with promoter sequences displaying differential expression profiles of B-class MADS-box genes for phalaenopsis floral morphogenesis.
      Hsu, CC; Wu, PS; Chen, TC; Yu, CW; Tsai, WC; Wu, K; Wu, WL; Chen, WH; Chen, HH
      PloS one  9  e106033  2014

      Show Abstract
      25501842 25501842
      G9a is transactivated by C/EBPβ to facilitate mitotic clonal expansion during 3T3-L1 preadipocyte differentiation.
      Li, SF; Guo, L; Qian, SW; Liu, Y; Zhang, YY; Zhang, ZC; Zhao, Y; Shou, JY; Tang, QQ; Li, X
      American journal of physiology. Endocrinology and metabolism  304  E990-8  2013

      Show Abstract
      Western Blotting23512806 23512806

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      Life Science Research > Antibodies and Assays > Primary Antibodies