07-213 | Anti-dimethyl-Histone H4 (Arg3) Antibody

07-213
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Vrt, InvWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-213
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H4 (Arg3) Antibody
      Alternate Names
      • H4 histone family, member A
      • histone 1
      • H4a
      • histone cluster 1
      • H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Acid extracted proteins from HeLa cells.
      PresentationPurified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, 5 mg/mL BSA and 30% glycerol.
      Applications
      ApplicationAnti-dimethyl-Histone H4 (Arg3) Antibody is a Rabbit Polyclonal Antibody for detection of dimethyl-Histone H4 (Arg3) also known as H4 histone family member A, histone 1 H4a & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesMethylation/Western Blot Assay: Reported by an independent laboratory to detect Histone H4 methylated in vitro by recombinant PRMT1 and detected by western blot analysis.
      Biological Information
      ImmunogenBSA-conjugated synthetic peptide (SG[me2R]GKGGKG-C) corresponding to amino acids 1-9 of Histone H4 with a C-terminal cysteine added to facilitate conjugation. The immunizing sequence is conserved from Tetrahymena to human.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes methylated Histone H4 (Arg3), MW 10 kDa. Addition of 0.1% Tween®-20 in western blot buffers results in the detection of additional proteins at 17 kDa (possibly methylated Histone H2a), 34 kDa and 80 kDa. Specificity demonstrated by ELISA using methylated and unmethylated Histone H4 peptides. The antibody does not detect recombinant Histone H3 purified from E. coli.
      IsotypeIgG
      Species Reactivity
      • Human
      • Vertebrates
      • Invertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Methylation
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: A 1:500-1:2000 dilution of this antibody detected methylated Histone H4 in acid-extracted proteins from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H4 (Arg3) - 20450 20450
      Anti-dimethyl-Histone H4 (Arg3) - 22779 22779
      Anti-dimethyl-Histone H4 (Arg3) - 22974 22974
      Anti-dimethyl-Histone H4 (Arg3) - 24004 24004
      Anti-dimethyl-Histone H4 (Arg3) - 27735 27735
      Anti-dimethyl-Histone H4 (Arg3) - 29942 29942

      References

      Reference overviewApplicationSpeciesPub Med ID
      Acute running stimulates hippocampal dopaminergic neurotransmission in rats, but has no influence on brain-derived neurotrophic factor.
      Maaike Goekint,Inge Bos,Elsa Heyman,Romain Meeusen,Yvette Michotte,Sophie Sarre
      Journal of applied physiology (Bethesda, Md. : 1985)  112  2012

      Show Abstract
      22134693 22134693
      The HPV E6 oncoprotein targets histone methyltransferases for modulating specific gene transcription.
      Hsu, CH; Peng, KL; Jhang, HC; Lin, CH; Wu, SY; Chiang, CM; Lee, SC; Yu, WC; Juan, LJ
      Oncogene  31  2335-49  2012

      Show Abstract
      Western Blotting21963854 21963854
      Characterization of the PRMT gene family in rice reveals conservation of arginine methylation.
      Ahmad, A; Dong, Y; Cao, X
      PloS one  6  e22664  2011

      Show Abstract Full Text Article
      Western Blotting21853042 21853042
      Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
      Shechter, D; Nicklay, JJ; Chitta, RK; Shabanowitz, J; Hunt, DF; Allis, CD
      The Journal of biological chemistry  284  1064-74  2009

      Show Abstract
      18957438 18957438
      Functional connection between deimination and deacetylation of histones.
      Denis, H; Deplus, R; Putmans, P; Yamada, M; Métivier, R; Fuks, F
      Molecular and cellular biology  29  4982-93  2009

      Show Abstract
      19581286 19581286
      The protein arginine methyltransferases CARM1 and PRMT1 cooperate in gene regulation.
      Kleinschmidt, MA; Streubel, G; Samans, B; Krause, M; Bauer, UM
      Nucleic acids research  36  3202-13  2008

      Show Abstract Full Text Article
      18413343 18413343
      PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation.
      Hyllus, D; Stein, C; Schnabel, K; Schiltz, E; Imhof, A; Dou, Y; Hsieh, J; Bauer, UM
      Genes & development  21  3369-80  2007

      Show Abstract Full Text Article
      Human18079182 18079182
      hCAF1, a new regulator of PRMT1-dependent arginine methylation.
      Robin-Lespinasse, Y; Sentis, S; Kolytcheff, C; Rostan, MC; Corbo, L; Le Romancer, M
      Journal of cell science  120  638-47  2007

      Show Abstract
      17264152 17264152
      USF1 recruits histone modification complexes and is critical for maintenance of a chromatin barrier.
      Huang, S; Li, X; Yusufzai, TM; Qiu, Y; Felsenfeld, G
      Molecular and cellular biology  27  7991-8002  2007

      Show Abstract Full Text Article
      17846119 17846119
      MBD2/NuRD and MBD3/NuRD, two distinct complexes with different biochemical and functional properties.
      Le Guezennec, X; Vermeulen, M; Brinkman, AB; Hoeijmakers, WA; Cohen, A; Lasonder, E; Stunnenberg, HG
      Molecular and cellular biology  26  843-51  2006

      Show Abstract Full Text Article
      16428440 16428440

      FAQ

      QuestionAnswer
      Is this peptide asymmetric or symmetric?This is an asymmetric dimethyl-modified Histone H4 peptide.