07-031 | Anti-dimethyl-Histone H4 (Lys20) Antibody

07-031
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, WB, Mplex, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-031
      Replaces07-747
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H4 (Lys20) Antibody
      Alternate Names
      • H4K20me2
      • Histone H4 (di methyl K20)
      References
      Product Information
      FormatAffinity Purified
      Presentation0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
      Applications
      ApplicationAnti-dimethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of dimethyl-Histone H4 (Lys20) also known as H4K20me2, Histone H4 (di methyl K20) & has been validated in ChIP, IF, WB, Mplex, ICC.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Multiplexing
      • Immunocytochemistry
      Biological Information
      ImmunogenPeptide corresponding to amino acids 17-28 of human histone H4 (RHRdimKVLRDNIQG-C) with lysine 20 dimethylated.
      HostRabbit
      Specificityrecognizes Histone H4 dimethylated on Lysine 20
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Methylation
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid-extracted HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H4 (Lys20) - 20299 20299
      Anti-dimethyl-Histone H4 (Lys20) - 24799 24799

      References

      Reference overviewApplicationPub Med ID
      A cellular study of teosinte Zea mays subsp. parviglumis (Poaceae) caryopsis development showing several processes conserved in maize.
      Marina Dermastia,Ales Kladnik,Jasna Dolenc Koce,Prem S Chourey
      American journal of botany  96  2009

      Show Abstract
      21622300 21622300
      An in vitro investigation of herbs traditionally used for kidney and urinary system disorders: potential therapeutic and toxic effects.
      Ken Wojcikowski,Hans Wohlmuth,David W Johnson,Margaret Rolfe,Glenda Gobe
      Nephrology (Carlton, Vic.)  14  2009

      Show Abstract
      18808387 18808387
      The fission yeast Jmj2 reverses histone H3 Lysine 4 trimethylation.
      Huarte, M; Lan, F; Kim, T; Vaughn, MW; Zaratiegui, M; Martienssen, RA; Buratowski, S; Shi, Y
      The Journal of biological chemistry  282  21662-70  2007

      Show Abstract
      Western Blotting17550896 17550896
      Profile of histone lysine methylation across transcribed mammalian chromatin.
      Vakoc, CR; Sachdeva, MM; Wang, H; Blobel, GA
      Molecular and cellular biology  26  9185-95  2006

      Show Abstract
      17030614 17030614
      Granulocyte heterochromatin: defining the epigenome.
      Olins, DE; Olins, AL
      BMC cell biology  6  39  2005

      Show Abstract Full Text Article
      16287503 16287503

      FAQ

      QuestionAnswer
      What is the concentration of this antibody?The presence of BSA in this antibody limits our ability to perform a Bradford Concentration assay.