05-672 | Anti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4

05-672
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-672
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4
      Alternate Names
      • H4K20me2
      • Histone H4 (di methyl K20)
      References
      Product Information
      FormatPurified
      PresentationPBS
      Applications
      ApplicationUse Anti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4 (mouse monoclonal antibody) validated in IP, WB, IHC to detect dimethyl-Histone H4 (Lys20) also known as H4K20me2, Histone H4 (di methyl K20).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      Immunogenpeptide corresponding to amino acids 17-28 of human histone H4 (RHRdimKVLRDNIQGC) dimethylated on lysine 20.
      Cloneclone 6G7/H4
      HostMouse
      Specificitymethyl-Histone H4
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Methylation
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa acid extracts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2138533 2138533
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2384632 2384632
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 1990542 1990542
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 1995610 1995610
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2066050 2066050
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2280286 2280286
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 -2536303 2536303
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 -2580689 2580689
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 -2601715 2601715
      Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 -2739443 2739443

      References

      Reference overviewPub Med ID
      RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.
      Frédérick A Mallette,Francesca Mattiroli,Gaofeng Cui,Leah C Young,Michael J Hendzel,Georges Mer,Titia K Sixma,Stéphane Richard
      The EMBO journal  31  2012

      Show Abstract
      22373579 22373579
      PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin.
      Nishioka, Kenichi, et al.
      Mol. Cell, 9: 1201-13 (2002)  2002

      Show Abstract
      12086618 12086618