07-1382 | Anti-eEF2 (CT) Antibody

07-1382
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, IHC, FC, IPRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1382
      DescriptionAnti-eEF2 (CT) Antibody
      Alternate Names
      • eukaryotic translation elongation factor 2
      • polypeptidyl-tRNA translocase
      Background InformationeEF2 (eukaryotic translation elongation factor 2) is essential for protein synthesis. It promotes the GTP-dependent translocation during peptide chain elongation. eEF2 is phosphorylated and inhibited by eEF2 kinase (eEF2K) on Thr56. eEF2K activity is inhibited by phosphorylation by both mTOR and p70S6K. This in turn up regulates eEF2 activity and protein synthesis. Similarly, AMPK down regulates protein synthesis by inhibited the activity of both p70S6K and mTOR, through TSC2.
      References
      Product Information
      FormatUnpurified
      Control
      • HEK293 cell lysate.
      PresentationUnpurified rabbit polyclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationThis Anti-eEF2 (C-term) Antibody is validated for use in WB, IC, IH, FC, IP for the detection of eEF2 (C-term).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesImmunohistochemistry:
      1:100 – 1:250 dilution of a previous lot was used on immunohistochemistry.

      Immunocytochemistry:
      1:50 – 1:100 dilution of a previous lot was used on immunocytochemistry.

      Immunoprecipitation:
      1:50 dilution of a previous lot was used on immunoprecipitation.

      Flow Cytometry:
      1:20 dilution of a previous lot was used on flow cytometry.

      Biological Information
      ImmunogenKLH conjugated linear peptide.
      EpitopeC-terminus
      HostRabbit
      SpecificityThis antibody recognizes eEf2 at the C-Terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteProven to react with human, mouse and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the
      ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
      Gene Symbol
      • EEF-2
      • EF-2
      • EF2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.

      LOCATION: Cytoplasm.

      PTM: Phosphorylation by EF-2 kinase completely inactivates EF-2.

      Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.

      SIMILARITY: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

      Molecular Weight95 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HEK293 cell lysate.

      Western Blot Analysis:
      A 1:50,000 dilution of this lot was to detect eEF2 on HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications