04-347 | Anti-eIF-4E CT Antibody, rabbit monoclonal

04-347
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, IP, WB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-347
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-eIF-4E CT Antibody, rabbit monoclonal
      Alternate Names
      • D-eIF4E
      • Eif4e
      • IF4E
      • deIF4E
      • eIF-4E1
      • eIF-4E2
      • eIF-4EII
      • eIF-4e
      References
      Product Information
      FormatPurified
      Presentation100 μL of rabbit monoclonal IgG in 30 mM Tris-Glycine (pH 7.4), 90 mM NaCl, 0.006% sodium azide, 0.03% BSA and 40% glycerol.
      Applications
      ApplicationDetect eIF-4E CT using this Anti-eIF-4E CT Antibody, Rabbit validated for use in FC, IP, WB, IH(P).
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesSuggested Dilutions:
      Immunoblot Analysis: 1:20,000
      Immunoprecipitation: 1:140
      Flow Cytometry: 1:40
      Immunohistochemistry: 1:250-1:500
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to residues in the C-term of human eIF-4E.
      HostRabbit
      SpecificityRecognizes the C-terminal domain of eIF-4E.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAll eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]
      Gene Symbol
      • EIF4E
      • eIF4E
      • eIF-4E
      • EIF-4E
      • EIF4E1
      • CBP
      • EIF4EL1
      • MGC111573
      • EIF4F
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06730 # Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
      SIZE: 217 amino acids; 25097 Da
      SUBUNIT: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP- kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 and EIF4A2. Interacts with NGDN (By similarity). Interacts with Lassa virus Z protein.
      PTM: Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
      SIMILARITY: SwissProt: P06730 ## Belongs to the eukaryotic initiation factor 4E family.
      Molecular Weight25 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-eIF-4E CT (Rabbit monoclonal IgG) - 2150696 2150696
      Anti-eIF-4E CT (Rabbit monoclonal IgG) - 2386996 2386996
      Anti-eIF-4E CT (Rabbit monoclonal IgG) - 2445817 2445817
      Anti-eIF-4E CT (Rabbit monoclonal IgG) - 2197144 2197144
      Anti-eIF-4E CT (Rabbit monoclonal IgG) - 2208752 2208752
      Anti-eIF-4E CT (Rabbit monoclonal IgG) - 2276276 2276276
      Anti-eIF-4E CT (Rabbit monoclonal IgG) - DAM1460233 DAM1460233
      Anti-eIF-4E CT - DAM1514045 DAM1514045
      Anti-eIF-4E CT - DAM1745400 DAM1745400
      Anti-eIF-4E CT - NG1840115 NG1840115

      References

      Reference overviewPub Med ID
      Eukaryotic initiation factor 4E variants alter the morphology, proliferation, and colony-formation properties of MDA-MB-435 cancer cells
      Goldson, Tove M, et al
      Mol Carcinog, 46:71-84 (2007)  2007

      17091471 17091471
      4E-binding protein 1, a cell signaling hallmark in breast cancer that correlates with pathologic grade and prognosis.
      Rojo, Federico, et al.
      Clin. Cancer Res., 13: 81-9 (2007)  2007

      Show Abstract
      17200342 17200342
      Expression levels of eIF4E, VEGF, and cyclin D1, and correlation of eIF4E with VEGF and cyclin D1 in multi-tumor tissue microarray
      Yang, Sherry X, et al
      Oncol Rep, 17:281-7 (2007)  2007

      17203162 17203162
      Cap-free structure of eIF4E suggests a basis for conformational regulation by its ligands
      Volpon, Laurent, et al
      EMBO J, 25:5138-49 (2006)  2006

      17036047 17036047

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation