05-478 | Anti-erbB-4/HER-4 Antibody, clone H4.72.8

05-478
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, NEUTMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-478
      Replaces04-348
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-erbB-4/HER-4 Antibody, clone H4.72.8
      References
      Product Information
      FormatPurified
      Presentation10mM PBS, pH 7.4
      Applications
      ApplicationThis Anti-erbB-4/HER-4 Antibody, clone H4.72.8 is validated for use in FC, IP, NEUT for the detection of erbB-4/HER-4.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Neutralizing
      Application NotesNot recommended for western blot.
      Biological Information
      ImmunogenExtracellular domain of recombinant human c-erbB-4/HER-4
      Cloneclone H4.72.8
      HostMouse
      Specificityc-erbB-4/HER-4 but not EGF receptor, c-erbB-2/HER-2, or c-erbB-3/HER-3
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
      Gene Symbol
      • ERBB3
      • c-erbB-3
      • c-erbB3
      • erbB-3
      • p180-ErbB3
      • p85-sErbB3
      • p45-sErbB3
      • ErbB-3
      • erbB3-S
      • MGC88033
      • HER3
      • MDA-BF-1
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P21860 # Binds and is activated by neuregulins and NTAK.
      SIZE: 1342 amino acids; 148098 Da
      SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 and PA2G4.
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Epithelial tissues and brain.
      DOMAIN: SwissProt: P21860 The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
      PTM: Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (By similarity).
      DISEASE: SwissProt: P21860 # Overexpressed in a subset of human mammary tumors. & Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      Molecular Weight185kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitationof c-erbB4/HER 4 from heregulin stimulated MDA-MB453 cells, and subsequently immunobloted with anti-Phosphotyrosine (Catalog #05-321)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-erbB-4/HER-4, clone H4.72.8 2428514
      Anti-erbB-4/HER-4, clone H4.72.8 (mouse monoclonal IgG2a) - 1988575 1988575
      Anti-erbB-4/HER-4, clone H4.72.8 (mouse monoclonal IgG2a) - 2281616 2281616
      Anti-erbB-4/HER-4, clone H4.72.8 (mouse monoclonal IgG2a) - 2322017 2322017
      Anti-erbB-4/HER-4, clone H4.72.8 (mouse monoclonal IgG2a) -2491093 2491093
      Anti-erbB-4/HER-4, clone H4.72.8 - 0608038458 0608038458
      Anti-erbB-4/HER-4, clone H4.72.8 - 16795 16795
      Anti-erbB-4/HER-4, clone H4.72.8 - 2492089 2492089
      Anti-erbB-4/HER-4, clone H4.72.8 - 31964 31964
      Anti-erbB-4/HER-4, clone H4.72.8 - 32439 32439

      References

      Reference overviewPub Med ID
      Engineered bivalent ligands to bias ErbB receptor-mediated signaling and phenotypes.
      Jay, SM; Kurtagic, E; Alvarez, LM; de Picciotto, S; Sanchez, E; Hawkins, JF; Prince, RN; Guerrero, Y; Treasure, CL; Lee, RT; Griffith, LG
      The Journal of biological chemistry  286  27729-40  2011

      Show Abstract
      21622572 21622572
      An immunological approach reveals biological differences between the two NDF/heregulin receptors, ErbB-3 and ErbB-4.
      Chen, X, et al.
      J. Biol. Chem., 271: 7620-9 (1996)  1996

      8631797 8631797

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies