04-291 | Anti-erbB2 (intracellular domain) Antibody, clone 24B5

04-291
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-291
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-erbB2 (intracellular domain) Antibody, clone 24B5
      Alternate Names
      • ERBB2
      References
      Product Information
      FormatPurified
      Control
      • EGF treated SKOV-3 lysates
      Presentationlyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
      Applications
      ApplicationAnti-erbB2 (intracellular domain) Antibody, clone 24B5 detects level of erbB2 (intracellular domain) & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of the intracellular domain in human erbB2
      Clone24B5
      HostMouse
      SpecificityRecognizes the intracellular domain of erbB2 (aa 860-880).
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
      Gene Symbol
      • ERBB2
      • HER2
      • HER-2
      • NGL
      • herstatin
      • erbB-2
      • C-erbB-2
      • NEU
      • TKR1
      • p185erbB2
      • HER-2/neu
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
      SIZE: 1255 amino acids; 137910 Da
      SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
      SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      Molecular Weight185 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-erbB2 (intracellular domain), clone 24B/5 (Mouse monoclonal IgG) 2934598
      Anti-erbB2 (intracellular domain), clone 24B5 - 2053913 2053913
      Anti-erbB2 (intracellular domain), clone 24B5 - DAM1480174 DAM1480174
      Anti-erbB2 (intracellular domain), clone 24B5 - DAM1519007 DAM1519007
      Anti-erbB2 (intracellular domain), clone 24B5 - DAM1713262 DAM1713262
      Anti-erbB2 (intracellular domain), clone 24B5 -2542044 2542044
      Anti-erbB2 (intracellular domain), clone 24B5 -2607886 2607886
      Anti-erbB2 (intracellular domain), clone 24B5 -2647546 2647546

      References

      Reference overviewPub Med ID
      Epidermal growth factor receptor inhibition strategies in pancreatic cancer: past, present and the future
      Cohenuram, Michael and Saif, Muhammad Wasif
      JOP, 8:4-15 (2007)  2007

      17228128 17228128
      Removal of the membrane-anchoring domain of epidermal growth factor leads to intracrine signaling and disruption of mammary epithelial cell organization.
      Wiley, H S, et al.
      J. Cell Biol., 143: 1317-28 (1998)  1998

      Show Abstract
      9832559 9832559

      Brochure

      Title
      Western Blotting Tools

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies