06-1293 | Anti-hCMT1 Antibody

06-1293
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBShSerumPolyclonal Antibody
      Description
      Catalogue Number06-1293
      DescriptionAnti-hCMT1 Antibody
      Alternate Names
      • RNA (guanine-7-) methyltransferase
      • mRNA (guanine-7-)methyltransferase
      • mRNA (guanine-N(7)-)-methyltransferase
      • mRNA cap methyltransferase
      Background InformationhCMT1 or mRNA (guanine-N(7)-)-methyltransferase belongs to the mRNA cap methyltransferase family. It is widely expressed across various tissues and is located in the nucleus. hCMT1 fuctions as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. It also binds RNA containing 5'-terminal GpppC. hCMT1 interacts with importin alpha, leading to the stimulation of both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization. Importin beta dissociating in response to RanGTP allows RNMT-importin alpha to bind RNA substrates. hCMT1 also interacts with elongating form of polymerase II and RNGTT.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell lysate
      PresentationSheep polyclonal serum containing 0.05% sodium azide.
      Applications
      ApplicationAnti-hCMT1 Antibody is a Sheep Polyclonal Antibody for detection of hCMT1 also known as mRNA (guanine-7-)methyltransferase & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A 1:1,000 dilution from a previous lot detected hCMT1 in 10 µg of HeLa cell lysate.
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to full length hCMT1.
      HostSheep
      SpecificityThis antibody recognizes hCMT1.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Mouse and rat (77% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DKFZp686H1252
      • EC 2.1.1.56
      • KIAA0398
      • MET
      • OTTHUMP00000162509
      • RG7MT1
      • hCMT1
      • hCMT1c
      • hMet
      • hcm1p
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNTION: mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.
      CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
      SUBUNIT STRUCTURE: Interacts with importin alpha, leading to stimulate both RNA-binding and methyltransferase activity. Interaction with importin alpha and beta is required for its nuclear localization, importin beta dissociating in response to RanGTP, allowing RNMT-importin alpha to bind RNA substrates. Interacts with elongating form of polymerase II and RNGTT.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Widely expressed.
      SEQUENCE SIMILARITIES: Belongs to the mRNA cap methyltransferase family.
      Molecular Weight~55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
      Western Blot Analysis: A 1:1,000 dilution of this antibody detected hCMT1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies