07-624 | Anti-hSNF2H Antibody

07-624
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-624
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-hSNF2H Antibody
      OverviewThe SNF2 family of proteins are ATP dependant chromatin remodeling enzymes. The gene encoding this protein is also known as SMARCA5
      References
      Product Information
      FormatAffinity Purified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect hSNF2H with Anti-hSNF2H Antibody (Rabbit Polyclonal Antibody), that has been shown to work in ChIP, IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to amino acids 58-77 (MEEIFDDASPGKQKEIQEPD) of human hSNF2H (SWI/SNF-related matrix-associated actin-dependent chromatin a5)
      HostRabbit
      SpecificityhSNF2H
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryThe protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
      Gene Symbol
      • SMARCA5
      • WCRF135
      • hISWI
      • hSNF2H
      • SNF2H
      • HSNF2H
      • ISWI
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60264 # Possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin in vitro; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter.
      SIZE: 1052 amino acids; 121905 Da
      SUBUNIT: Catalytic subunit of the four known chromatin-remodeling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. ACF/WCRF contains BAZ1A; CHRAC contains BAZ1A, CHRAC1, and POLE3; RSF contains RSF1; WICH contains BAZ1B. SMARCA5 is the catalytic subunit of the NoRC chromatin-remodeling complex, which also contains BAZ2A and of SMARCA5/cohesin/NuRD complexes. The BAZ2A subunit of NoRC also interacts with DNMT1, DNMT3B and HDAC1, which allows NoRC to actively suppress rDNA transcription by a combination of nucleosome remodeling, histone deacetylation, and DNA methylation.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitously expressed.DEVELOPMENTAL STAGE: Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.
      SIMILARITY: SwissProt: O60264 ## Belongs to the SNF2/RAD54 helicase family. ISWI subfamily. & Contains 1 helicase ATP-binding domain. & Contains 1 helicase C-terminal domain. & Contains 2 SANT domains.
      Molecular Weight120kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in nuclear extracts from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-hSNF2H - 27070 27070

      References

      Reference overviewPub Med ID
      A chromatin remodelling complex that loads cohesin onto human chromosomes.
      Hakimi, Mohamed-Ali, et al.
      Nature, 418: 994-8 (2002)  2002

      Show Abstract
      12198550 12198550

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies