05-698 | Anti-hSNF2h Antibody, clone 1B9/D12

05-698
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-698
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-hSNF2h Antibody, clone 1B9/D12
      OverviewThe SNF2 family of proteins are ATP dependant chromatin remodeling enzymes. Anti-hSNF2, clone 1B9/D12 recognizes both hSNF2H and hSNF2L
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationAnti-hSNF2h Antibody, clone 1B9/D12 is a high quality Mouse Monoclonal Antibody for the detection of hSNF2h & has been validated in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenPurified, full-length human SNF2h
      Cloneclone 1B9/D12
      HostMouse
      SpecificitySNF2h, aka SMARCA5 and SNF2L, aka SMARCA1
      IsotypeIgG3
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.
      Gene Symbol
      • SMARCA5
      • WCRF135
      • hISWI
      • hSNF2H
      • SNF2H
      • HSNF2H
      • ISWI
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60264 # Possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin in vitro; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter.
      SIZE: 1052 amino acids; 121905 Da
      SUBUNIT: Catalytic subunit of the four known chromatin-remodeling complexes: CHRAC, RSF, ACF/WCRF, and WICH. Each complex contains subunits which may regulate the specificity or catalytic activity of SMARCA5. ACF/WCRF contains BAZ1A; CHRAC contains BAZ1A, CHRAC1, and POLE3; RSF contains RSF1; WICH contains BAZ1B. SMARCA5 is the catalytic subunit of the NoRC chromatin-remodeling complex, which also contains BAZ2A and of SMARCA5/cohesin/NuRD complexes. The BAZ2A subunit of NoRC also interacts with DNMT1, DNMT3B and HDAC1, which allows NoRC to actively suppress rDNA transcription by a combination of nucleosome remodeling, histone deacetylation, and DNA methylation.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitously expressed.DEVELOPMENTAL STAGE: Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.
      SIMILARITY: SwissProt: O60264 ## Belongs to the SNF2/RAD54 helicase family. ISWI subfamily. & Contains 1 helicase ATP-binding domain. & Contains 1 helicase C-terminal domain. & Contains 2 SANT domains.
      Molecular Weight135kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on nuclear extract from HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-hSNF2h, clone 1B9/D12 - 24347 24347

      References

      Reference overviewApplicationPub Med ID
      Runx1 regulation of Pu.1 corepressor/coactivator exchange identifies specific molecular targets for leukemia differentiation therapy.
      Gu, X; Hu, Z; Ebrahem, Q; Crabb, JS; Mahfouz, RZ; Radivoyevitch, T; Crabb, JW; Saunthararajah, Y
      The Journal of biological chemistry  289  14881-95  2014

      Show Abstract
      24695740 24695740
      Loss of WSTF results in spontaneous fluctuations of heterochromatin formation and resolution, combined with substantial changes to gene expression.
      Culver-Cochran, AE; Chadwick, BP
      BMC genomics  14  740  2013

      Show Abstract
      24168170 24168170
      The WSTF-ISWI chromatin remodeling complex transiently associates with the human inactive X chromosome during late S-phase prior to BRCA1 and γ-H2AX.
      Culver-Cochran, AE; Chadwick, BP
      PloS one  7  e50023  2012

      Show Abstract
      23166813 23166813
      Chromatin remodeling complex NURF regulates thymocyte maturation.
      Landry, JW; Banerjee, S; Taylor, B; Aplan, PD; Singer, A; Wu, C
      Genes & development  25  275-86  2011

      Show Abstract Full Text Article
      21289071 21289071
      A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
      Kobor, MS; Venkatasubrahmanyam, S; Meneghini, MD; Gin, JW; Jennings, JL; Link, AJ; Madhani, HD; Rine, J
      PLoS biology  2  E131  2004

      Show Abstract Full Text Article
      Western Blotting15045029 15045029
      Reconstitution of recombinant chromatin establishes a requirement for histone-tail modifications during chromatin assembly and transcription.
      Loyola, A, et al.
      Genes Dev., 15: 2837-51 (2001)  2001

      Show Abstract
      11691835 11691835
      Purification and characterization of a human factor that assembles and remodels chromatin.
      LeRoy, G, et al.
      J. Biol. Chem., 275: 14787-90 (2000)  2000

      Show Abstract
      10747848 10747848

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies