05-1522 | Anti-hnRNP A2/B1 Antibody, clone DP383

05-1522
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1522
      DescriptionAnti-hnRNP A2/B1 Antibody, clone DP383
      Alternate Names
      • heterogeneous nuclear ribonucleoprotein A2/B1
      • heterogeneous nuclear ribonucleoprotein B1
      • hnRNP A2 / hnRNP B1
      • nuclear ribonucleoprotein particle A2 protein
      Background InformationThe hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. While all hnRNPs are present in the nucleus, data suggests they shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by hnRNP A2/B1 belongs to the A/B subfamily of ubiquitously express hnRNPs. This family member has two repeats of quasi-RRM domains that bind to RNAs. Two alternatively spliced transcript variants which encode different isoforms have been described. In autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, and mixed connective tissue disease hnRNPA2B1 can serve as an autoantigen. Although this protein is predominantly nucleoplasmic, in some tissues isoform A2 is found in the cytoplasm.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-hnRNP A2/B1 Antibody, clone DP383 (Mouse Monoclonal Antibody) validated in WB, IHC, IP to detect hnRNP A2/B1 also known as heterogeneous nuclear ribonucleoprotein A2/B1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry:
      Cited in Mizukami, K., et al. (2000) Neuroreport. 11(14):3099-102 using a representative lot of this antibody.
      Immunoprecipitation:
      Cited in Kamma, H., et al. (1999) Exp. Cell Res. 246(2):399-411using a representative lot of this antibody.
      Biological Information
      ImmunogenRecombinant protein corresponding to human hnRNP A2/B1.
      EpitopeUnknown
      CloneDP383
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes hnRNP A2/B1.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms. [provided by RefSeq].
      Gene Symbol
      • HNRNPA2
      • HNRNPB1
      • HNRPA2
      • HNRPA2B1
      • HNRPB1
      • RNPA2
      • SNRPB1
      • HNRNPA2B1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous.

      SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1.

      SUBCELLULAR LOCATION: Nucleus › nucleoplasm. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues. Not found in the nucleolus. Ref.15

      SEQUENCE SIMILARITIES: Contains 2 RRM (RNA recognition motif) domains.
      Molecular Weight~ 36/38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected hnRNP A2/B1 on 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications