06-1354 | Anti-mRNA-capping enzyme Antibody

06-1354
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1354
      DescriptionAnti-mRNA-capping enzyme Antibody
      Alternate Names
      • mRNA-capping enzyme
      • HCAP1
      • HCE
      • Polynucleotide 5'-triphosphatase
      • mRNA 5'-triphosphatase
      • Tpase
      • mRNA guanylyltransferase
      • GTP--RNA guanylyltransferase
      • GTase
      Background InformationmRNA capping enzyme is one of the first mRNA transcription cycle factors to be associated with the C-terminal domain (CTD) and functions by adding an inverted guanylyl group (also known as a ‘cap’) to the 5’ end. mRNA capping enzyme also has triphosphatase and guanylyltransferase activity. The CTD also serves to regulate the capping enzyme and has the ability to remove the Ser5 phosphate which is linked to the release of the capping enzyme from the capping complex. mRNA capping enzyme has a variety of other functions during transcription including; stabilization of polymerase II, activation of early elongation and reduction of re-initiation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa nuclear extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-mRNA-capping enzyme Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect mRNA-capping enzyme also known as mRNA-capping enzyme, HCAP1, HCE.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected mRNA-capping enzyme in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human mRNA-capping enzyme.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNGTT
      • CAP1A
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.

      CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.

      GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

      SUBUNIT STRUCTURE: Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Nucleus (By similarity).

      TISSUE SPECIFICITY: Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.

      MISCELLANEOUS: Isoform 2 to isoform 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTase domain.

      SEQUENCE SIMILARITIES: In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.

      In the C-terminal section; belongs to the eukaryotic GTase family.
      Molecular Weight~66 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 1:5,000 dilution of this antibody detected mRNA-capping enzyme on 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies