04-385 | Anti-mTOR Antibody, rabbit monoclonal

04-385
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, ICC, IH(P), IP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-385
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-mTOR Antibody, rabbit monoclonal
      Alternate Names
      • FRAP1
      • FRAP
      • FRAP2
      • RAFT1
      • RAPT1
      • FK506 binding protein 12-rapamycin associated protein 1
      Background InformationmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
      References
      Product Information
      FormatCulture Supernatant
      Presentation100 μL of rabbit monoclonal IgG in 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-mTOR Antibody is validated for use in FC, IC, IH(P), IP, WB for the detection of mTOR.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to the C-terminal region of human mTOR.
      HostRabbit
      SpecificityRecognizes a region in the C-terminus domain of human mTOR.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene.
      Gene Symbol
      • FRAP1
      • RAPT1
      • MTOR
      • FRAP
      • mTOR
      • FLJ44809
      • FRAP2
      • RAFT1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42345 # Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
      SIZE: 2549 amino acids; 288892 Da
      SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex.
      TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis.
      SIMILARITY: SwissProt: P42345 ## Belongs to the PI3/PI4-kinase family. & Contains 1 FAT domain. & Contains 1 FATC domain. & Contains 7 HEAT repeats. & Contains 1 PI3K/PI4K domain.
      Molecular Weight289 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-mTOR - 2145047 2145047
      Anti-mTOR - 2428507 2428507
      Anti-mTOR - 2006914 2006914
      Anti-mTOR - 2067218 2067218
      Anti-mTOR - 2147036 2147036
      Anti-mTOR - 2298346 2298346
      Anti-mTOR - 2562325 2562325
      Anti-mTOR - NG1767540 NG1767540
      Anti-mTOR - NG1855854 NG1855854
      Anti-mTOR - NG1873278 NG1873278

      References

      Reference overviewApplicationPub Med ID
      AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role.
      Chen, KT; Tsai, MH; Wu, CH; Jou, MJ; Wei, IH; Huang, CC
      Frontiers in behavioral neuroscience  9  162  2015

      Show Abstract
      26150775 26150775
      Sirtuin 1 in rat orthotopic liver transplantation: an IGL-1 preservation solution approach.
      Pantazi, E; Zaouali, MA; Bejaoui, M; Folch-Puy, E; Ben Abdennebi, H; Varela, AT; Rolo, AP; Palmeira, CM; Roselló-Catafau, J
      World journal of gastroenterology  21  1765-74  2015

      Show Abstract
      25684941 25684941
      Conversion to sirolimus ameliorates cyclosporine-induced nephropathy in the rat: focus on serum, urine, gene, and protein renal expression biomarkers.
      Sereno, J; Nunes, S; Rodrigues-Santos, P; Vala, H; Rocha-Pereira, P; Fernandes, J; Santos-Silva, A; Teixeira, F; Reis, F
      BioMed research international  2014  576929  2014

      Show Abstract
      Immunohistochemistry24971338 24971338
      Inhibition of tumor cell growth, proliferation and migration by X-387, a novel active-site inhibitor of mTOR.
      Si-meng Chen,Jia-li Liu,Xiang Wang,Chris Liang,Jian Ding,Ling-hua Meng
      Biochemical pharmacology  83  2012

      Show Abstract
      22305748 22305748
      Epigallocatechin gallate (EGCG), a major component of green tea, is a dual phosphoinositide-3-kinase/mTOR inhibitor.
      Van Aller GS, Carson JD, Tang W, Peng H, Zhao L, Copeland RA, Tummino PJ, Luo L
      Biochem Biophys Res Commun  406  194-9. Epub 2011 Feb 15.  2011

      Show Abstract
      21300025 21300025
      SWI/SNF chromatin remodeling enzyme ATPases promote cell proliferation in normal mammary epithelial cells.
      Cohet, N; Stewart, KM; Mudhasani, R; Asirvatham, AJ; Mallappa, C; Imbalzano, KM; Weaver, VM; Imbalzano, AN; Nickerson, JA
      Journal of cellular physiology  223  667-78  2010

      Show Abstract
      Western Blotting20333683 20333683
      Safety analysis of the diaphragm in combination with lubricant or acidifying microbicide gels: effects on markers of inflammation and innate immunity in cervicovaginal fluid.
      Deborah J Anderson,D'Nyce L Williams,Susan A Ballagh,Kurt Barnhart,Mitchell D Creinin,Daniel R Newman,Frederick P Bowman,Joseph A Politch,Ann C Duerr,Denise J Jamieson
      American journal of reproductive immunology (New York, N.Y. : 1989)  61  2009

      Show Abstract
      19143675 19143675

      Posters

      Title
      Histone Modifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies