04-735 | Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal

04-735
100 µl  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, MplexRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-735
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314, rabbit monoclonal
      Alternate Names
      • H4K20me1
      • Histone H4 (mono methyl K20)
      • histone 4
      • H4
      • histone H4
      • histone cluster 4
      • H4
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Trimethylation of histone H4 on lys20 (H4-K20me3) selectively marks constitutive pericentromeric hetero-chromatin and imprinting control regions, while mono- and dimethylation are broadly distributed but principally enriched in euchromatin regions. Although the biological function of H4K20me remains poorly understood, studies suggest that H4-K20me2 is involved in the guidance of DNA repair proteins to DNA strand breaks, whereas H4K20me1 is associated with chromatin condensation processes. PR-Set7 (also known as SET8) specifically catalyzes monomethylation of H4K20me1.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HeLa acid extracts
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationUse Anti-monomethyl-Histone H4 (Lys20) Antibody, clone NL314 (rabbit monoclonal antibody) validated in WB, Mplex to detect monomethyl-Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20).
      Key Applications
      • Western Blotting
      • Multiplexing
      Application NotesImmunoblot Analysis:
      A 1:4000 dilution of a previous lot detected methylated histone H3 in acid extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.

      Multiplexing:
      Beadlyte® Histone-Peptide Specificity Assay: 1:2,000-1:30,000 dilutions of a previous lot were incubated with histone H4 peptides containing various modifications conjugated to Luminex® microspheres. Specificity to monomethyl-histone H4 (Lys20) was demonstrated (Figure C).
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence …HR[mmK]V…, in which mmK corresponds to monomethyl-lysine at residue 20 of human histone H4.
      CloneNL314
      HostRabbit
      SpecificityRecognizes monomethyl-histone H4 (Lys20), Mr ~10kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. The immunizing sequence is conserved from Tetrahymena to human, so broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Methylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight10kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis.
      Immunoblot Analysis:
      A 1:1000-1:2000 dilution of this lot detected monomethyl histone H4 (Lys20) in HeLa acid extracts. (Figure A).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-monomethyl-Histone H4 (Lys20), clone NL314 2470963
      Anti-monomethyl-Histone H4 (Lys20), clone NL314 - 2420411 2420411
      Anti-monomethyl-Histone H4 (Lys20), clone NL314 - 2341918 2341918
      Anti-monomethyl-Histone H4 (Lys20), clone NL314 - 2365995 2365995
      Anti-monomethyl-Histone H4 (Lys20), clone NL314 - DAM1563394 DAM1563394
      Anti-monomethyl-Histone H4 (Lys20), clone NL314 -2851183 2851183
      Anti-monomethyl-Histone H4 (Lys20), clone NL314 -2855890 2855890
      Anti-monomethyl-Histone H4 (Lys20),clone NL314 -2834548 2834548

      References

      Reference overviewPub Med ID
      Histone H4 lysine 20 of Saccharomyces cerevisiae is monomethylated and functions in subtelomeric silencing.
      Edwards, CR; Dang, W; Berger, SL
      Biochemistry  50  10473-83  2011

      Show Abstract
      21985125 21985125
      Certain and progressive methylation of histone H4 at lysine 20 during the cell cycle.
      Pesavento, James J, et al.
      Mol. Cell. Biol., 28: 468-86 (2008)  2008

      17967882 17967882
      L3MBTL1, a histone-methylation-dependent chromatin lock.
      Trojer, Patrick, et al.
      Cell, 129: 915-28 (2007)  2007

      Show Abstract
      17540172 17540172
      A trans-tail histone code defined by monomethylated H4 Lys-20 and H3 Lys-9 demarcates distinct regions of silent chromatin.
      Sims, Jennifer K, et al.
      J. Biol. Chem., 281: 12760-6 (2006)  2006

      16517599 16517599
      Specificity and mechanism of the histone methyltransferase Pr-Set7.
      Xiao, Bing, et al.
      Genes Dev., 19: 1444-54 (2005)  2005

      15933069 15933069

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.
      Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

      Posters

      Title
      Histone Modifications