06-500 | Anti-p130 Cas Antibody

06-500
250 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-500
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p130 Cas Antibody
      Overviewhas been referred to as anti-Cas3
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide
      Applications
      ApplicationDetect p130 Cas using this Anti-p130 Cas Antibody validated for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenGST-fusion protein containing amino acids 691-799 of rat p130 Cas
      HostRabbit
      Specificityp130 Cas
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BCAR1
      • CAS
      • p130CAS
      • Crkas
      • P130Cas
      • CRKAS
      • p130cas
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P56945 # Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
      SIZE: 870 amino acids; 93362 Da
      SUBUNIT: Forms complexes in vivo with focal adhesion kinase 1, adapter protein CRKL and LYN kinase. Can heterodimerize with CASL. Interacts with BCAR3, NPHP1, PTK2B and SH2D3C (By similarity). Interacts with activated CSPG4. Interacts with INPPL1/SHIP2.
      SUBCELLULAR LOCATION: Cell junction, focal adhesion (By similarity). Cytoplasm (By similarity). Note=Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation (By similarity).
      TISSUE SPECIFICITY: Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
      DOMAIN: "SwissProt: P56945 Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2- binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL (By similarity). & A serine-rich region promotes activation of the serum response element (SRE). & The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.
      PTM: Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin mediated adhesion of cells to the extracellular matrix.
      SIMILARITY: Belongs to the CAS family. & Contains 1 SH3 domain.
      Molecular Weight130kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates of human A431 carcinoma cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p130 Cas - 14370 14370
      Anti-p130 Cas - 19950 19950
      Anti-p130 Cas - 27649 27649
      Anti-p130 Cas - DAM1797933 DAM1797933

      References

      Reference overviewApplicationPub Med ID
      The emerin-binding transcription factor Lmo7 is regulated by association with p130Cas at focal adhesions.
      Wozniak, Michele A, et al.
      PeerJ, 1: e134 (2013)  2013

      Show Abstract
      24010014 24010014
      Activation of platelet-activating factor receptor and pleiotropic effects on tyrosine phospho-EGFR/Src/FAK/paxillin in ovarian cancer.
      Margarita Aponte, Wei Jiang, Montaha Lakkis, Ming-Jiang Li, Dale Edwards, Lina Albitar, Allison Vitonis, Samuel C Mok, Daniel W Cramer, Bin Ye
      Cancer research  68  5839-48  2008

      Show Abstract Full Text Article
      18632638 18632638
      CD36 signals to the actin cytoskeleton and regulates microglial migration via a p130Cas complex.
      Stuart, LM; Bell, SA; Stewart, CR; Silver, JM; Richard, J; Goss, JL; Tseng, AA; Zhang, A; El Khoury, JB; Moore, KJ
      The Journal of biological chemistry  282  27392-401  2007

      Show Abstract
      Western Blotting17623670 17623670
      Laminin alpha 3 forms a complex with beta3 and gamma3 chains that serves as the ligand for alpha 6beta1-integrin at the apical ectoplasmic specialization in adult rat testes.
      Yan, HH; Cheng, CY
      The Journal of biological chemistry  281  17286-303  2006

      Show Abstract
      16608848 16608848
      Regulation of ectoplasmic specialization dynamics in the seminiferous epithelium by focal adhesion-associated proteins in testosterone-suppressed rat testes.
      Wong, CH; Xia, W; Lee, NP; Mruk, DD; Lee, WM; Cheng, CY
      Endocrinology  146  1192-204  2005

      Show Abstract
      Immunofluorescence15591141 15591141
      Activation of platelet-activating factor receptor-coupled G alpha q leads to stimulation of Src and focal adhesion kinase via two separate pathways in human umbilical vein endothelial cells.
      Deo, DD; Bazan, NG; Hunt, JD
      The Journal of biological chemistry  279  3497-508  2004

      Show Abstract
      14617636 14617636
      A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner.
      Sakai, R, et al.
      EMBO J., 13: 3748-56 (1994)  1994

      Show Abstract
      8070403 8070403

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MABT165 Anti-p130cas/BCAR1 Antibody, clone 2C1.1 Show Pricing & Availability

      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies