05-257 | Anti-p300 CT Antibody, clone RW128

05-257
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-257
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p300 CT Antibody, clone RW128
      Alternate Names
      • KAT3B
      Background Informationp300 (also EP300 or E1A binding protein p300) and CBP (also CREBBP or CREB binding protein) are two closely related transcriptional co-activating proteins (or coactivators). p300 and CBP interact with numerous transcription factors and act to increase the expression of their target genes.
      p300 and CBP have similar structures. Both contain five protein interaction domains, the nuclear receptor interaction domain (RID), the CREB and MYB interaction domain (KIX), the cysteine/histidine regions (CH1 and CH3) and the interferon response binding domain (IBiD). In addition p300 and CBP each contain a protein or histone acetyltransferase (PAT/HAT) domain and a bromodomain that binds acetylated lysines and a PHD finger motif with unknown function.
      p300 and CBP are thought to increase gene expression in three ways:
      (1) by relaxing the chromatin structure at the gene promoter through their intrinsic histone acetyltransferase (HAT) activity
      (2) by recruiting the basal transcriptional machinery including RNA polymerase II to the promoter
      (3) by acting as adaptor molecules.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: HeLa Nuclear Extract (12-309)
      PresentationProtein A Purified immunoglobulin in Protein A Purified immunoglobulin in PBS, pH 7.4, 0.05% sodium azide and 30% glycerol
      Applications
      ApplicationUse Anti-p300 CT Antibody, clone RW128 (Mouse Monoclonal Antibody) validated in ChIP, IP, WB to detect p300 CT also known as KAT3B.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Biological Information
      ImmunogenGST-fusion to the C-Terminus of human p300
      EpitopeC-terminus
      CloneRW128
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes p300 and co-immunoprecipitates E1A.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryEP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CBP (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CBP can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
      Gene Symbol
      • EP300
      • p300
      • P300
      • OTTHUMP00000028668
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q09472 # Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
      SIZE: 2414 amino acids; 264144 Da
      SUBUNIT: Interacts with phosphorylated CREB1 (By similarity). Interacts with DTX1, EID1, ELF3, FEN1, LEF1, NCOA1, NCOA6, NR3C1, PCAF, PELP1, SPIB, SRY, TCF7L2, TP53, SRCAP and TRERF1. The TAZ- type 1 domain interacts with HIF1A. Probably part of a complex with HIF1A and CREBBP. Part of a complex containing CARM1 and NCOA2/GRIP1. Interacts with ING4 and this interaction may be indirect. Interacts with the C-terminal region of CITED4. Interacts with HTLV-1 Tax and p30II. Interacts with and acetylates HIV-1 Tat.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated. & Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1. & Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.
      DISEASE: SwissProt: Q09472 # Defects in EP300 may play a role in epithelial cancer. & Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with MYST3. & Defects in EP300 are a cause of Rubinstein-Taybi syndrome (RSTS) [MIM:180849]. RSTS is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
      SIMILARITY: SwissProt: Q09472 ## Contains 1 bromo domain. & Contains 1 KIX domain. & Contains 2 TAZ-type zinc fingers. & Contains 1 ZZ-type zinc finger.
      Molecular Weight300 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p300 CT, clone RW128 - 2430529 2430529
      Anti-p300 CT, clone RW128 - 2436414 2436414
      Anti-p300 CT, clone RW128 - 2446844 2446844
      Anti-p300 CT, clone RW128 - 13293 13293
      Anti-p300 CT, clone RW128 - 17574 17574
      Anti-p300 CT, clone RW128 - 18749 18749
      Anti-p300 CT, clone RW128 - 1967299 1967299
      Anti-p300 CT, clone RW128 - 2017304 2017304
      Anti-p300 CT, clone RW128 - 2031383 2031383
      Anti-p300 CT, clone RW128 - 2075962 2075962

      References

      Reference overviewApplicationSpeciesPub Med ID
      Mechanistic analysis of the role of bromodomain-containing protein 4 (BRD4) in BRD4-NUT oncoprotein-induced transcriptional activation.
      Wang, R; You, J
      The Journal of biological chemistry  290  2744-58  2015

      Show Abstract
      Immunofluorescence25512383 25512383
      DOT1L cooperates with the c-Myc-p300 complex to epigenetically derepress CDH1 transcription factors in breast cancer progression.
      Cho, MH; Park, JH; Choi, HJ; Park, MK; Won, HY; Park, YJ; Lee, CH; Oh, SH; Song, YS; Kim, HS; Oh, YH; Lee, JY; Kong, G
      Nature communications  6  7821  2015

      Show Abstract
      26199140 26199140
      MAF1 represses CDKN1A through a Pol III-dependent mechanism.
      Lee, YL; Li, YC; Su, CH; Chiao, CH; Lin, IH; Hsu, MT
      eLife  4  e06283  2015

      Show Abstract
      26067234 26067234
      An amino terminal phosphorylation motif regulates intranuclear compartmentalization of Olig2 in neural progenitor cells.
      Meijer, DH; Sun, Y; Liu, T; Kane, MF; Alberta, JA; Adelmant, G; Kupp, R; Marto, JA; Rowitch, DH; Nakatani, Y; Stiles, CD; Mehta, S
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  8507-18  2014

      Show Abstract
      Western Blotting24948806 24948806
      An H2A histone isotype regulates estrogen receptor target genes by mediating enhancer-promoter-3'-UTR interactions in breast cancer cells.
      Su, CH; Tzeng, TY; Cheng, C; Hsu, MT
      Nucleic acids research  42  3073-88  2014

      Show Abstract
      24371278 24371278
      Otx2 and Oct4 drive early enhancer activation during embryonic stem cell transition from naive pluripotency.
      Yang, SH; Kalkan, T; Morissroe, C; Marks, H; Stunnenberg, H; Smith, A; Sharrocks, AD
      Cell reports  7  1968-81  2014

      Show Abstract
      24931607 24931607
      Src is activated by the nuclear receptor peroxisome proliferator-activated receptor β/δ in ultraviolet radiation-induced skin cancer.
      Montagner, A; Delgado, MB; Tallichet-Blanc, C; Chan, JS; Sng, MK; Mottaz, H; Degueurce, G; Lippi, Y; Moret, C; Baruchet, M; Antsiferova, M; Werner, S; Hohl, D; Saati, TA; Farmer, PJ; Tan, NS; Michalik, L; Wahli, W
      EMBO molecular medicine  6  80-98  2014

      Show Abstract
      Western Blotting24203162 24203162
      Three-dimensional collagen I promotes gemcitabine resistance in vitro in pancreatic cancer cells through HMGA2-dependent histone acetyltransferase expression.
      Dangi-Garimella, S; Sahai, V; Ebine, K; Kumar, K; Munshi, HG
      PloS one  8  e64566  2013

      Show Abstract
      23696899 23696899
      Up-regulation of ciliary neurotrophic factor in astrocytes by aspirin: implications for remyelination in multiple sclerosis.
      Modi, KK; Sendtner, M; Pahan, K
      The Journal of biological chemistry  288  18533-45  2013

      Show Abstract
      23653362 23653362
      Involvement of p300/CBP and epigenetic histone acetylation in TGF-β1-mediated gene transcription in mesangial cells.
      Yuan, H; Reddy, MA; Sun, G; Lanting, L; Wang, M; Kato, M; Natarajan, R
      American journal of physiology. Renal physiology  304  F601-13  2013

      Show Abstract
      23235480 23235480

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

      Related Products & Applications

      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies