07-503 | Anti-p40-phox Antibody

07-503
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-503
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p40-phox Antibody
      References
      Product Information
      FormatPurified
      PresentationProtein A purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
      Applications
      ApplicationAnti-p40-phox Antibody is an antibody against p40-phox for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenC-terminal 6HIS-tagged fusion protein corresponding to full length human p40-phox
      HostRabbit
      Specificityp40-phox
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • NCF4
      • SH3PXD4
      • p40-phox
      • OTTHUMP00000043756
      • MGC3810
      • NCF
      • P40PHOX
      • p40phox
      • OTTHUMP00000028736
      • NCF-4
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15080 # Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
      SIZE: 339 amino acids; 39032 Da
      SUBUNIT: p40-PHOX associates primarily with p67-PHOX to form a complex with p47-PHOX.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expression is restricted to hematopoietic cells.
      SIMILARITY: SwissProt: Q15080 ## Contains 1 PX (phox homology) domain. & Contains 1 SH3 domain.
      Molecular Weight40kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot in RIPA lysates of HL-60 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p40-phox (rabbit polyclonal IgG) 2860345
      Anti-p40-phox (rabbit polyclonal IgG) 2899920
      Anti-p40-phox (rabbit polyclonal IgG) 2876659
      Anti-p40-phox - 24600 24600
      Anti-p40-phox - 3045054 3045054

      References

      Reference overviewPub Med ID
      A fluorescently tagged C-terminal fragment of p47phox detects NADPH oxidase dynamics during phagocytosis.
      Li, XJ; Tian, W; Stull, ND; Grinstein, S; Atkinson, S; Dinauer, MC
      Molecular biology of the cell  20  1520-32  2009

      Show Abstract Full Text Article
      19129478 19129478
      Regulation of renal epithelial tight junctions by the von Hippel-Lindau tumor suppressor gene involves occludin and claudin 1 and is independent of E-cadherin.
      Harten SK, Shukla D, Barod R, Hergovich A, Balda MS, Matter K, Esteban MA, Maxwell PH.
      Molecular biology of the cell  20  1089-101  2001

      Show Abstract Full Text Article
      19073886 19073886
      p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains.
      Wientjes, F B, et al.
      Biochem. J., 296 ( Pt 3): 557-61 (1993)  1993

      Show Abstract
      8280052 8280052