05-393 | Anti-p80 Ku Antibody, clone GE2.9.5

05-393
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-393
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-p80 Ku Antibody, clone GE2.9.5
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide
      Applications
      ApplicationAnti-p80 Ku Antibody, clone GE2.9.5 is a high quality Mouse Monoclonal Antibody for the detection of p80 Ku & has been validated in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenT-cell protein complex containing Ku.
      Cloneclone GE2.9.5
      HostMouse
      Specificityp80 subunit of the Ku autoantigen
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
      Gene Symbol
      • XRCC5
      • Ku86
      • KARP-1
      • NFIV
      • Ku80
      • TLAA
      • CTC85
      • CTCBF
      • G22P2
      • KARP1
      • FLJ39089
      • KU80
      • KUB2
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13010 # Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NARG1, the Ku p70/p86 dimer binds to the osteocalcin promoter and activates osteocalcin expression.
      SIZE: 732 amino acids; 82705 Da
      SUBUNIT: Heterodimer of a 70 kDa and a 80 kDa subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA- dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NARG1, and this complex displays DNA binding activity towards the osteocalcin FGF response element (OCFRE). In addition, the 80 kDa subunit binds to the osteoblast-specific transcription factors MSX2 and RUNX2. Interacts with ELF3. May interact with APLF.
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P13010 The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
      PTM: Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. & Sumoylated.DISEASE:SwissProt: P13010 # Individuals with systemic lupus erythematosus (SLE) and related disorders produce extremely large amounts of autoantibodies to p70 and p86.
      SIMILARITY: Contains 1 Ku domain.
      Molecular Weight80kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on a HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-p80 Ku, clone GE2.9.5 - 15391 15391
      Anti-p80 Ku, clone GE2.9.5 - 18430 18430
      Anti-p80 Ku, clone GE2.9.5 - 25201 25201

      References

      Reference overviewApplicationPub Med ID
      A gene expression signature identifying transient DNMT1 depletion as a causal factor of cancer-germline gene activation in melanoma.
      Cannuyer, J; Van Tongelen, A; Loriot, A; De Smet, C
      Clinical epigenetics  7  114  2015

      Show Abstract
      26504497 26504497
      Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint.
      Mulvey, CM; Tudzarova, S; Crawford, M; Williams, GH; Stoeber, K; Godovac-Zimmermann, J
      Journal of proteome research  12  1436-53  2013

      Show Abstract
      Western Blotting23320540 23320540
      DNA-dependent ATPase from HeLa cells is related to human Ku autoantigen.
      Cao, Q P, et al.
      Biochemistry, 33: 8548-57 (1994)  1994

      Show Abstract
      8031790 8031790
      Assembly and DNA binding of recombinant Ku (p70/p80) autoantigen defined by a novel monoclonal antibody specific for p70/p80 heterodimers.
      Wang, J, et al.
      J. Cell. Sci., 107 ( Pt 11): 3223-33 (1994)  1994

      Show Abstract
      7699019 7699019
      Human DNA helicase II: a novel DNA unwinding enzyme identified as the Ku autoantigen.
      Tuteja, N, et al.
      EMBO J., 13: 4991-5001 (1994)  1994

      Show Abstract
      7957065 7957065
      Antigenic determinants of the 70-kDa subunit of the Ku autoantigen.
      Abu-Elheiga, L and Yaneva, M
      Clin. Immunol. Immunopathol., 64: 145-52 (1992)  1992

      1379527 1379527

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies