04-292 | Anti-phosho-erbB2/Her2 (Ser1113) Antibody, clone 9E10

04-292
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-292
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phosho-erbB2/Her2 (Ser1113) Antibody, clone 9E10
      Alternate Names
      • NEU
      • NGL
      • HER2
      • TKR1
      • HER-2
      • c-erb B2
      • HER-2/neu
      References
      Product Information
      FormatPurified
      Control
      • Pervanadate treated A431 cell lysates
      PresentationLyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
      Applications
      ApplicationThis Anti-phosho-erbB2/Her2 (Ser1113) Antibody, clone 9E10 is validated for use in ELISA, WB for the detection of phosho-erbB2/Her2 (Ser1113).
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Ser 1113 in human erbB2/Her2
      Clone9E10
      HostMouse
      SpecificityRecognizes phosphorylated domain of erbB2/Her2 on Serine 1113
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
      Gene Symbol
      • ERBB2
      • HER2
      • HER-2
      • NGL
      • herstatin
      • erbB-2
      • C-erbB-2
      • NEU
      • TKR1
      • p185erbB2
      • HER-2/neu
      Modifications
      • Phosphorylation
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04626 # Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF- alpha and amphiregulin.
      SIZE: 1255 amino acids; 137910 Da
      SUBUNIT: Heterodimer with each of the other ERBB receptors (Potential). Interacts with PRKCABP and PLXNB1. Part of a complex with EGFR and either PIK3C2A or PIK3C2B. May interact with PIK3C2B when phosphorylated on Tyr-1196. Interacts with MEMO when phosphorylated on Tyr-1248.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Ligand-binding increases phosphorylation on tyrosine residues (By similarity).
      SIMILARITY: SwissProt: P04626 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      Molecular Weight185 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phosho-erbB2/Her2 (Ser1113), clone 9E10 (Mouse monoclonal IgG1) - 0139S0101 0139S0101
      Anti-phosho-erbB2/Her2 (Ser1113), clone 9E10 - DAM1546240 DAM1546240

      References

      Reference overviewPub Med ID
      The duration of phorbol-inducible ErbB2 tyrosine dephosphorylation parallels that of receptor endocytosis rather than threonine-686 phosphorylation: implications for the physiological role of protein kinase C in growth factor receptor signalling
      Ouyang, X, et al
      Carcinogenesis, 19:2013-9 (1998)  1998

      9855018 9855018
      Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity
      Ouyang, X, et al
      J Biol Chem, 271:21786-92 (1996)  1996

      8702975 8702975

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies