04-199 | Anti-phospho-ATF-2 (Thr71) Antibody, clone E268, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, ICCRbAscitesMonoclonal Antibody
      Catalogue Number04-199
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-ATF-2 (Thr71) Antibody, clone E268, rabbit monoclonal
      Alternate Names
      • activating transcription factor 2
      • activating transcription factor 2 splice variant ATF2-var2
      • cAMP response element-binding protein CRE-BP1
      • cAMP responsive element binding protein 2
      • cAMP responsive element binding protein 2
      • formerly
      Background InformationThe ATF/CREB family of DNA binding proteins consists of transcription factors whose activity is regulated by specific signaling pathways, including the stress/cytokine/radiation inducible pathways that function through SAPK1/JNK activation. Two threonine residues at positions 69 and 71 in the N-terminal region of ATF-2 are essential in mediating adenovirus E1A-inducible transcriptional activation. These residues are efficiently phosphorylated in vivo and are excellent substrates for SAPK1/JNK in vitro.
      Product Information
      • Untreated and Calyculin/Okadaic acid treated HeLa Cell Lysate.
      PresentationUnpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-phospho-ATF-2 (Thr71) Antibody, clone E268, Rabbit is validated for use in IP, WB, IC for the detection of phospho-ATF-2 (Thr71).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunofluorescent Immunocytochemistry:

      A previous lot of this antibody was used in IP.
      Biological Information
      ImmunogenSynthetic peptide directed against the region encompassing and including phosphorylated ATF2 (Thr71).
      SpecificityThis antibody recognizes phosphorylated residues surrounding Thr71 of human ATF-2.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with Human. Not expected to react with rat and mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. [provided by RefSeq].
      Gene Symbol
      • CRE-BP1
      • CREB2
      • CREBP1
      • HB16
      • MGC111558
      • OTTHUMP00000163262
      • TREB7
      • Phosphorylation
      Non-Reactive Species
      • Mouse
      • Rat
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION:Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

      SUBUNIT: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Ref.5


      SPECIFICITY: Abundant expression seen in the brain.

      PTM: Phosphorylation of Thr-69 and Thr-71 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK. Ref.6 Ref.7 Ref.8 Ref.9

      SIMILARITY: Belongs to the bZIP family. ATF subfamily.

      Contains 1 bZIP domain.

      Contains 1 C2H2-type zinc finger.

      CAUTION: It is uncertain whether Met-1 or Met-19 is the initiator.

      Molecular Weight70 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on untreated and Anisomycin treated HeLa cell lysate.

      Western Blot Analysis:
      A 1:2500-1:5000 dilution of this lot was used to detect ATF-2 in untreated and Anisomycin treated HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information