05-891 | Anti-phospho-ATF2 (Thr69/71) Antibody, clone AW65

05-891
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-891
      Replaces04-199
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-ATF2 (Thr69/71) Antibody, clone AW65
      References
      Product Information
      FormatPurified
      Applications
      ApplicationAnti-phospho-ATF2 (Thr69/71) Antibody, clone AW65 is a high quality Mouse Monoclonal Antibody for the detection of phospho-ATF2 (Thr69/71) & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence ...DQ[pT]P[pT]P... in which [pT] corresponds to phospho-threonine at residues 69 and 71 of human ATF2
      CloneAW65
      HostMouse
      SpecificityATF2 phosphorylated at both Thr69 and Thr71
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NotePredicted to cross-react with rat based on sequence homology
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
      Gene Symbol
      • ATF2
      • MGC111558
      • CREB2
      • TREB7
      • CRE-BP1
      • CREBP1
      • HB16
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P15336 # Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.
      SIZE: 487 amino acids; 52277 Da
      SUBUNIT: Binds DNA as a dimer and can form a homodimer in the absence of DNA. Can form a heterodimer with JUN. Interacts with SMAD3 and SMAD4. Binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Abundant expression seen in the brain.
      PTM: Phosphorylation of Thr-51 and Thr-53 by MAPK14 causes increased transcriptional activity. Also phosphorylated and activated by JNK.
      SIMILARITY: SwissProt: P15336 ## Belongs to the bZIP family. ATF subfamily. & Contains 1 bZIP domain. & Contains 1 C2H2-type zinc finger.
      Molecular WeightMr 65kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot with RIPA lysates from anisomycin treated 3T3/NIH cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditionsstable 2 years at -20°C from date of shipment
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-phospho-ATF2 (Thr69/71), clone AW65 - 2487901 2487901
      Anti-phospho-ATF2 (Thr69/71), clone AW65 - 30345 30345
      Anti-phospho-ATF2 (Thr69/71), clone AW65 - JBC1915862 JBC1915862

      References

      Reference overviewPub Med ID
      TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2.
      Delhase, M; Kim, SY; Lee, H; Naiki-Ito, A; Chen, Y; Ahn, ER; Murata, K; Kim, SJ; Lautsch, N; Kobayashi, KS; Shirai, T; Karin, M; Nakanishi, M
      Proceedings of the National Academy of Sciences of the United States of America  109  E177-86  2012

      Show Abstract
      22203995 22203995
      Growth factor stimulation induces cell survival by c-Jun. ATF2-dependent activation of Bcl-XL.
      Salameh, A; Galvagni, F; Anselmi, F; De Clemente, C; Orlandini, M; Oliviero, S
      The Journal of biological chemistry  285  23096-104  2010

      Show Abstract Full Text Article
      20507983 20507983

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies