05-740-AF488 | Anti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488

05-740-AF488
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-740-AF488
      DescriptionAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488
      Alternate Names
      • Serine-protein kinase ATM, Ser1981-phosphorylated
      • Ataxia telangiectasia mutated, Ser1981-phosphorylated
      • A-T mutated, Ser1981-phosphorylated
      • ATM, Ser1981-phosphorylated
      Background InformationSerine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Applications
      ApplicationAnti-phospho-ATM (Ser1981) Antibody, clone 10H11.E12, Alexa Fluor® 488 is an antibody against phospho-ATM (Ser1981) for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThe unconjugated antibody (Cat. Nos. 05-740, MAB3806 & MAB3806-C) is shown to be suitable also for ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting applications. PE-conjugate (Cat. No. FCMAB110P) is also available and tested for Flow cytometry application.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence is 11/12 identical to the corresponding mouse and rat sequence.
      EpitopepSer1981.
      Clone10H11.E12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityReacts with ATM phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ATM
      • AT
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      UniProt Number
      Molecular WeightThe unconjugated antibody (Cat. No. 05-430) is shown to recognize the ~370 kDa phosphoATM target band and a >400 kDa non-specific band.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected UV-induced ATM Ser1981 phosphorylation in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies