07-2129 | Anti-phospho-Abi-1 (Ser225) Antibody

07-2129
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Mk, H, M, RWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2129
      DescriptionAnti-phospho-Abi-1 (Ser225) Antibody
      Alternate Names
      • Abl interactor 1
      • Abelson interactor 1
      • Abi-1
      • Abl-binding protein 4
      • AblBP4
      • Eps8 SH3 domain-binding protein
      • Eps8-binding protein
      • Nap1-binding protein
      • Nap1BP
      • Spectrin SH3 domain-binding protein 1
      • e3B1
      Background InformationAbl interactor 1 (Abelson interactor 1) is a member of the family of Abl-interactors and is highly expressed in the brain. Abl interactor 1 is involved in the signaling of ABL1 and ABL2 nonreceptor tyrosine kinases, and the EGF-receptor. The Abl interactor 1 protein contains SH3 domains, and may function as an adaptor protein; It forms a trimeric complex with EPS8 and SOS1 in the transduction of signals from Ras to Rac. Previous studies have suggested that Abl interactor 1 via the Ras and Rac pathways may regulate cell proliferation, cell adhesion, and actin remodeling. Abl interactor 1 is also investigated for its role in tumor suppression.
      References
      Product Information
      FormatAffinity Purified
      Control
      • EGF-treated COS cells untreated and treated with lambda phosphatase.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis phospho-Abi-1 antibody is validated for use in WB for the detection of the phospho-Abi-1 protein.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A representative lot was used by an independent laboratory in human mammary cells treated with EGF and Abi-1-transfected 293T cells (Mendoza, M., et al. (2011). Molecular Cell 41:661–671).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Abi-1 phosphorylated at Ser225.
      EpitopePhosphorylated Ser225
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Abi-1 phosphorylated at Ser225.
      Species Reactivity
      • Monkey
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Monkey and Human.
      Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, the encoded protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • ABI1
      • SSH3BP1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level (By similarity).

      SUBUNIT STRUCTURE: Interacts with ABL1, ENAH, STX1A, SNAP25, VAMP2, EPS8, and through its N-terminus with WASF1. Part of a complex consisting of ABI1, STX1A and SNAP25. Part of a complex consisting of ABI1, EPS8 and SOS1 (By similarity). Interacts with SOS1, SOS2, GRB2, SPTA1 and the first SH3 domain of NCK1. Isoform 6 does not interact with NCK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2.

      SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus (By similarity). Cell projection › lamellipodium (By similarity). Cell projection › filopodium (By similarity). Cell projection › growth cone (By similarity). Cell junction › synapse › synaptosome (By similarity). Cytoplasm › cytoskeleton (By similarity). Note: Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes (By similarity).

      TISSUE SPECIFICTY: Widely expressed, with highest expression in brain.

      DOMAIN: The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A (By similarity).

      PTM: In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.

      INVOLVEMENT IN DISEASE: Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.

      SEQUENCE SIMILARITIES: Belongs to the ABI family.

      Contains 1 SH3 domain.

      Contains 1 t-SNARE coiled-coil homology domain.
      Molecular Weight~60 kDa observed.
      55 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in EGF-treated COS cells untreated and treated with lambda phosphatase.

      Western Blot Analysis: 1 µg/mL of this antibody detected Abi-1 in 10 µg of EGF-treated COS cells untreated and treated with lambda phosphatase.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies